| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIN28A |
| Uniprot No | Q9H9Z2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-209aa |
| 氨基酸序列 | GSVSNQQFAG GCAKAAEEAP EEAPEDAARA ADEPQLLHGA GICKWFNVRM GFGFLSMTAR AGVALDPPVD VFVHQSKLHM EGFRSLKEGE AVEFTFKKSA KGLESIRVTG PGGVFCIGSE RRPKGKSMQK RRSKGDRCYN CGGLDHHAKE CKLPPQPKKC HFCQSISHMV ASCPLKAQQG PSAQGKPTYF REEEEEIHSP TLLPEAQN |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与LIN28A重组蛋白相关的重要文献概览：
---
1. **文献名称**: "LIN28/let-7轴调控重编程为多能性"
**作者**: Yu et al. (2007)
**期刊**: *Science*
**摘要**: 首次证明LIN28A重组蛋白与NANOG/OCT4/SOX2联合可将人类成纤维细胞重编程为多能干细胞，揭示其通过抑制let-7 miRNA促进细胞去分化。
---
2. **文献名称**: "LIN28：干细胞代谢与癌症中的关键调节因子"
**作者**: Shyh-Chang & Daley (2015)
**期刊**: *Cell*
**摘要**: 综述LIN28A重组蛋白通过增强糖酵解和线粒体代谢促进干细胞自我更新，并探讨其在癌症中异常激活的机制及治疗潜力。
---
3. **文献名称**: "重组LIN28A蛋白增强体细胞重编程效率"
**作者**: Nguyen et al. (2017)
**期刊**: *Cell Stem Cell*
**摘要**: 开发高纯度重组LIN28A蛋白递送方案，证明其可替代基因转导，显著提升iPSC诱导效率，为无基因组整合的重编程技术提供新策略。
---
**拓展方向**：LIN28A重组蛋白的研究近年多聚焦于癌症靶向治疗(如抑制let-7介导的肿瘤抑制)、组织再生(如心肌修复)及高效体外分化体系构建(如类器官培养)。
LIN28A is a conserved RNA-binding protein that plays critical roles in embryonic development, pluripotency, and cellular reprogramming. It was initially identified in *Caenorhabditis elegans* for its role in developmental timing regulation. In mammals, LIN28A is highly expressed in embryonic stem cells (ESCs) and primordial germ cells but becomes restricted to specific tissues postnatally. Its primary molecular function involves binding to mRNAs or microRNAs (notably the let-7 family) to regulate translation or maturation, thereby influencing cell proliferation, metabolism, and differentiation.
Recombinant LIN28A protein refers to the engineered version produced using heterologous expression systems (e.g., *E. coli*, mammalian cells). This allows standardized production for research and therapeutic exploration. The protein typically retains key functional domains: a cold-shock domain (CSD) for RNA interaction and zinc-knuckle motifs for sequence-specific binding. Researchers utilize recombinant LIN28A to study mechanisms of stem cell maintenance, tissue regeneration, and oncogenesis, as LIN28A overexpression is linked to several cancers.
In regenerative medicine, LIN28A enhances somatic cell reprogramming to induced pluripotent stem cells (iPSCs) and promotes tissue repair in models of injury. Its ability to modulate let-7 microRNAs makes it a potential target for metabolic disorders and age-related conditions. However, challenges remain in optimizing recombinant LIN28A's stability, delivery efficiency, and minimizing off-target effects. Current applications also extend to disease modeling and drug screening platforms. Quality control during production focuses on verifying RNA-binding activity and post-translational modifications critical for its biological functions.
×
