纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MYH8 |
Uniprot No | P13535 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 全长 |
氨基酸序列 | full |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYH8重组蛋白的3篇代表性文献概览(注:文献为示例性内容,实际引用需核对真实数据库):
1. **文献名称**: *"Functional characterization of MYH8 mutations in congenital myopathies using recombinant protein models"*
**作者**: Smith A, et al.
**摘要**: 本研究通过体外重组表达MYH8突变体蛋白,分析了其在肌动蛋白结合和ATP酶活性中的异常,揭示了MYH8突变导致先天性肌病的分子机制。
2. **文献名称**: *"Expression and purification of human MYH8 recombinant protein for structural studies"*
**作者**: Zhang L, et al.
**摘要**: 开发了一种高效的大肠杆菌表达系统,用于制备高纯度人源MYH8重组蛋白,并通过X射线晶体学解析其关键结构域的三维构象。
3. **文献名称**: *"MYH8-recombinant protein enhances cellular contractility in engineered muscle tissues"*
**作者**: Tanaka K, et al.
**摘要**: 在体外构建的工程化肌肉组织中,外源性添加MYH8重组蛋白显著增强了肌纤维的收缩能力,提示其在肌肉再生治疗中的潜在应用价值。
如需具体文献检索,建议通过PubMed或Web of Science以“MYH8 recombinant protein”为关键词查询最新研究。
MYH8. or myosin heavy chain 8. is a sarcomeric protein belonging to the myosin heavy chain (MyHC) family, which plays a critical role in muscle contraction. Encoded by the MYH8 gene in humans, it is specifically classified as a component of skeletal muscle myosin and is notably expressed during embryonic development and in certain specialized muscle tissues postnatally. MYH8 is also referred to as MyHC-perinatal due to its predominant expression in the late fetal and early postnatal stages, gradually replaced by other adult isoforms in most muscles. Structurally, MYH8 consists of a motor domain responsible for ATP hydrolysis and actin binding, a lever arm involved in force generation, and a tail region mediating filament assembly.
Mutations in MYH8 are associated with human myopathies, including trismus-pseudocamptodactyly syndrome and distal arthrogryposis type 2A, characterized by congenital joint contractures and muscle weakness. These disorders highlight MYH8's essential role in musculoskeletal development and function. Recombinant MYH8 protein is engineered to study its biochemical properties, interactions, and pathogenic mechanisms. Produced using expression systems like bacteria or mammalian cells, the recombinant protein retains functional domains for in vitro assays, such as actin motility or ATPase activity measurements. Researchers utilize it to investigate MYH8-related mutations, screen therapeutic compounds, and develop diagnostic tools. Its applications extend to understanding sarcomere dynamics, muscle disease pathology, and regenerative medicine. Overall, MYH8 recombinant protein serves as a vital tool for unraveling the molecular basis of myosin-driven processes and advancing therapeutic strategies for neuromuscular disorders.
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