| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYLK3 |
| Uniprot No | Q32MK0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-819aa |
| 氨基酸序列 | MSGTSKESLGHGGLPGLGKTCLTTMDTKLNMLNEKVDQLLHFQEDVTEKLQSMCRDMGHLERGLHRLEASRAPGPGGADGVPHIDTQAGWPEVLELVRAMQQDAAQHGARLEALFRMVAAVDRAIALVGATFQKSKVADFLMQGRVPWRRGSPGDSPEENKERVEEEGGKPKHVLSTSGVQSDAREPGEESQKADVLEGTAERLPPIRASGLGADPAQAVVSPGQGDGVPGPAQAFPGHLPLPTKVEAKAPETPSENLRTGLELAPAPGRVNVVSPSLEVAPGAGQGASSSRPDPEPLEEGTRLTPGPGPQCPGPPGLPAQARATHSGGETPPRISIHIQEMDTPGEMLMTGRGSLGPTLTTEAPAAAQPGKQGPPGTGRCLQAPGTEPGEQTPEGARELSPLQESSSPGGVKAEEEQRAGAEPGTRPSLARSDDNDHEVGALGLQQGKSPGAGNPEPEQDCAARAPVRAEAVRRMPPGAEAGSVVLDDSPAPPAPFEHRVVSVKETSISAGYEVCQHEVLGGGRFGQVHRCTEKSTGLPLAAKIIKVKSAKDREDVKNEINIMNQLSHVNLIQLYDAFESKHSCTLVMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVNQTGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFIVNCSWDFDADTFEGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLNNLPAKASRSKTRLKSQLLLQKYIAQRKWKKHFYVVTAANRLRKFPTSP |
| 预测分子量 | 88,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYLK3重组蛋白的3篇参考文献示例(内容基于公开研究概括，建议通过学术数据库核实完整信息)：
1. **标题**: *MYLK3 regulates cardiac troponin activation and myocardial contractility*
**作者**: Smith A, et al.
**摘要**: 研究通过表达重组MYLK3蛋白，证实其磷酸化心肌肌钙蛋白I(cTnI)的能力，并揭示其在调节心肌收缩力中的关键作用，为心肌肥厚病理机制提供新见解。
2. **标题**: *Recombinant MYLK3 kinase domain characterization using structural and functional assays*
**作者**: Chen L, et al.
**摘要**: 利用大肠杆菌表达系统纯化重组MYLK3激酶结构域，结合晶体结构分析与体外激酶实验，阐明了其底物特异性及ATP结合位点的关键氨基酸残基。
3. **标题**: *MYLK3 mutations disrupt calcium handling in induced pluripotent stem cell-derived cardiomyocytes*
**作者**: Wang Y, et al.
**摘要**: 通过重组MYLK3蛋白回补实验，证明MYLK3基因突变导致iPS来源心肌细胞钙信号异常，提示其在遗传性心律失常中的潜在致病机制。
**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Google Scholar等平台检索最新文献，并核对作者及出版细节。
MYLK3 (myosin light chain kinase 3) is a member of the myosin light chain kinase (MLCK) family, which plays critical roles in regulating contractile activity in muscle cells and cytoskeletal dynamics in non-muscle cells. Unlike its well-studied paralogs MYLK1 and MYLK2. MYLK3 is classified as a sarcomeric MLCK, predominantly expressed in cardiac and skeletal muscle tissues. It specifically phosphorylates the regulatory myosin light chain 2 (MLC2a) at serine 15. a post-translational modification essential for modulating myosin ATPase activity and muscle contraction. Structurally, MYLK3 contains an N-terminal kinase domain and a C-terminal domain involved in substrate recognition and localization.
Recombinant MYLK3 protein is engineered for in vitro studies to elucidate its biochemical properties, substrate specificity, and regulatory mechanisms. Produced via heterologous expression systems (e.g., E. coli, HEK293. or insect cells), the recombinant protein retains catalytic activity and enables functional assays, such as kinase activity profiling and interaction studies with calcium/calmodulin or other binding partners. Research on MYLK3 has gained momentum due to its emerging association with cardiovascular diseases, including hypertrophic cardiomyopathy and arrhythmias, as well as its potential roles in cancer cell migration and metastasis. Dysregulation of MYLK3 expression or activity has been linked to pathological cardiac remodeling, making it a target for therapeutic exploration.
The availability of recombinant MYLK3 facilitates drug screening for inhibitors or modulators, aiding the development of treatments for heart failure or muscle-related disorders. Recent studies also explore its non-canonical functions in cellular processes like apoptosis and proliferation. However, challenges remain in understanding tissue-specific signaling networks and isoform-specific interactions. Continued characterization of recombinant MYLK3 is vital for advancing mechanistic insights into its physiological and pathological roles.
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