| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYLK2 |
| Uniprot No | Q9H1R3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-596aa |
| 氨基酸序列 | ATENGAVEL GIQNPSTDKA PKGPTGERPL AAGKDPGPPD PKKAPDPPTL KKDAKAPASE KGDGTLAQPS TSSQGPKGEG DRGGGPAEGS AGPPAALPQQ TATPETSVKK PKAEQGASGS QDPGKPRVGK KAAEGQAAAR RGSPAFLHSP SCPAIISSSE KLLAKKPPSE ASELTFEGVP MTHSPTDPRP AKAEEGKNIL AESQKEVGEK TPGQAGQAKM QGDTSRGIEF QAVPSEKSEV GQALCLTARE EDCFQILDDC PPPPAPFPHR MVELRTGNVS SEFSMNSKEA LGGGKFGAVC TCMEKATGLK LAAKVIKKQT PKDKEMVLLE IEVMNQLNHR NLIQLYAAIE TPHEIVLFME YIEGGELFER IVDEDYHLTE VDTMVFVRQI CDGILFMHKM RVLHLDLKPE NILCVNTTGH LVKIIDFGLA RRYNPNEKLK VNFGTPEFLS PEVVNYDQIS DKTDMWSMGV ITYMLLSGLS PFLGDDDTET LNNVLSGNWY FDEETFEAVS DEAKDFVSNL IVKDQRARMN AAQCLAHPWL NNLAEKAKRC NRRLKSQILL KKYLMKRRWK KNFIAVSAAN RFKKISSSGA LMALGV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYLK2重组蛋白的3篇模拟参考文献(基于研究领域常见主题虚构，仅供参考)：
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1. **"Recombinant MYLK2 Expression and Its Role in Cardiac Hypertrophy Signaling"**
*Authors: Zhang, Y. et al.*
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组MYLK2蛋白，证实其具有特异性肌球蛋白轻链磷酸化活性。通过体外心肌细胞模型，发现MYLK2过表达通过激活钙调蛋白依赖通路促进病理性心肌肥厚。
2. **"Structural and Functional Characterization of MYLK2 Kinase Domain"**
*Authors: Smith, R.J. & Kumar, S.*
**摘要**: 利用昆虫细胞系统表达重组人源MYLK2激酶结构域，通过X射线晶体学解析其三维结构。功能实验表明，该结构域突变体(D158V)导致ATP结合能力下降，提示其在遗传性肌病中的潜在致病机制。
3. **"MYLK2 Recombinant Protein Enhances Skeletal Muscle Regeneration in Murine Models"**
*Authors: Chen, L. et al.*
**摘要**: 在CHO细胞中制备高纯度MYLK2重组蛋白，发现其能显著促进成肌细胞分化和迁移。动物实验显示局部注射该蛋白可加速损伤后骨骼肌修复，为肌肉再生治疗提供新策略。
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注：上述文献为示例性质，实际研究中请通过PubMed或Google Scholar检索真实文献(关键词如"MYLK2 recombinant protein"、"MYLK2 kinase activity")。真实文献可能集中于MYLK2的酶学特性、与肌病相关性(如远端关节挛缩症)或心脏功能调控方向。
**Background of MYLK2 Recombinant Protein**
Myosin light chain kinase 2 (MYLK2) is a calcium/calmodulin-dependent serine/threonine kinase that plays a critical role in regulating muscle contraction by phosphorylating the regulatory light chains of myosin. It is a member of the MYLK family, which includes isoforms expressed in smooth muscle (MYLK1) and non-muscle tissues (MYLK3/4). Unlike MYLK1. MYLK2 is predominantly expressed in striated muscles, particularly in cardiac and skeletal muscle tissues, where it fine-tunes contractile activity through its interaction with the actin-myosin apparatus.
The recombinant MYLK2 protein is engineered to enable detailed biochemical and functional studies. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains the enzymatic activity required for catalyzing myosin light chain phosphorylation. Researchers utilize this recombinant protein to investigate its kinase activity, substrate specificity, and regulatory mechanisms, particularly in contexts like cardiac hypertrophy, muscle development, or diseases linked to contractile dysfunction.
Recent studies highlight MYLK2's potential role beyond muscle contraction, including involvement in cell migration, cytoskeletal reorganization, and signaling pathways. Its dysregulation has been implicated in cardiomyopathies and skeletal muscle disorders, making it a target for therapeutic exploration. Additionally, recombinant MYLK2 serves as a tool for screening kinase inhibitors or modulators, aiding drug discovery efforts.
Structural studies using recombinant MYLK2 have also advanced understanding of its catalytic domain, calmodulin-binding regions, and autoinhibitory mechanisms. These insights are critical for designing targeted interventions. Overall, MYLK2 recombinant protein provides a versatile platform for unraveling the molecular basis of muscle physiology and pathology, bridging gaps between basic research and clinical applications.
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