首页 / 产品 / 蛋白 / 活性蛋白

Recombinant Human MYO5A protein

  • 中文名: 肌球蛋白ⅤA(MYO5A)重组蛋白
  • 别    名: MYO5A;MYH12;Unconventional myosin-Va
货号: PA1000-9783
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点MYO5A
Uniprot NoQ9Y4I1
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间全长
氨基酸序列full
预测分子量kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是与MYO5A重组蛋白相关的参考文献示例(文献信息为虚构,仅供格式参考):

1. **"Recombinant MYO5A expression and its role in melanosome transport"**

- **作者**: Chen et al.

- **摘要**: 研究通过昆虫细胞系统表达重组MYO5A蛋白,验证其与Rab27A的相互作用,揭示其在黑色素体运输中的分子机制。

2. **"Structural analysis of MYO5A motor domain using cryo-EM"**

- **作者**: Smith & Watanabe

- **摘要**: 利用重组MYO5A蛋白进行冷冻电镜结构解析,阐明其运动结构域在ATP水解过程中的构象变化。

3. **"In vitro reconstitution of MYO5A-driven cargo transport"**

- **作者**: Li et al.

- **摘要**: 通过重组MYO5A和微管蛋白体外重建实验,证明MYO5A在沿微管运输囊泡过程中的过程性运动能力。

4. **"Functional characterization of MYO5A mutations linked to Griscelli syndrome"**

- **作者**: Rodriguez et al.

- **摘要**: 表达携带致病突变的重组MYO5A蛋白,发现其与肌动蛋白结合能力缺陷,导致细胞运输功能障碍。

(注:以上文献为示例性质,实际研究中请通过PubMed或Web of Science等数据库检索真实文献。)

背景信息

MYO5A, a member of the myosin superfamily, is a motor protein critical for intracellular cargo transport. Classified as myosin V, it plays a key role in actin-based vesicular trafficking, organelle positioning, and cytoskeletal organization. Structurally, MYO5A consists of a motor domain (head) with ATPase activity for actin binding, a neck region with light chain-binding sites that regulate movement, and a tail domain responsible for cargo recognition. Its unique "walking" mechanism enables processive movement along actin filaments, distinguishing it from non-processive myosins.

Mutations in MYO5A are linked to Griscelli syndrome type 1 (GS1), characterized by partial albinism and neurological impairments due to defective melanosome transport and synaptic vesicle dynamics. Beyond GS1. MYO5A dysregulation is associated with cancer metastasis, neurodevelopmental disorders, and autoimmune conditions, highlighting its broad cellular impact.

Recombinant MYO5A proteins, typically produced in baculovirus or mammalian expression systems, retain post-translational modifications crucial for function. These engineered proteins enable detailed studies of motor mechanics, cargo-binding specificity (e.g., interactions with Rab GTPases), and pathological mutations. Researchers employ techniques like cryo-EM and single-molecule imaging to analyze conformational changes and force generation. Pharmaceutical applications include screening small molecules targeting MYO5A in diseases involving vesicular trafficking defects. Recent work explores its role in exosome secretion and immune cell function, expanding potential therapeutic targets. The recombinant protein serves as a vital tool for deciphering intracellular transport biology and developing precision interventions.

客户数据及评论

折叠内容

大包装询价

×