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Recombinant Human MYO1F protein

  • 中文名: 肌球蛋白ⅠF(MYO1F)重组蛋白
  • 别    名: MYO1F;Unconventional myosin-If
货号: PA1000-9781
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点MYO1F
Uniprot NoO00160
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-1098aa
氨基酸序列MGSKERFHWQSHNVKQSGVDDMVLLPQITEDAIAANLRKRFMDDYIFTYIGSVLISVNPFKQMPYFTDREIDLYQGAAQYENPPHIYALTDNMYRNMLIDCENQCVIISGESGAGKTVAAKYIMGYISKVSGGGEKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSRGGEPDGGKISNFLLEKSRVVMQNENERNFHIYYQLLEGASQEQRQNLGLMTPDYYYYLNQSDTYQVDGTDDRSDFGETLSAMQVIGIPPSIQQLVLQLVAGILHLGNISFCEDGNYARVESVDLLAFPAYLLGIDSGRLQEKLTSRKMDSRWGGRSESINVTLNVEQAAYTRDALAKGLYARLFDFLVEAINRAMQKPQEEYSIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIRWTPIQYFNNKVVCDLIENKLSPPGIMSVLDDVCATMHATGGGADQTLLQKLQAAVGTHEHFNSWSAGFVIHHYAGKVSYDVSGFCERNRDVLFSDLIELMQTSEQAFLRMLFPEKLDGDKKGRPSTAGSKIKKQANDLVATLMRCTPHYIRCIKPNETKRPRDWEENRVKHQVEYLGLKENIRVRRAGFAYRRQFAKFLQRYAILTPETWPRWRGDERQGVQHLLRAVNMEPDQYQMGSTKVFVKNPESLFLLEEVRERKFDGFARTIQKAWRRHVAVRKYEEMREEASNILLNKKERRRNSINRNFVGDYLGLEERPELRQFLGKRERVDFADSVTKYDRRFKPIKRDLILTPKCVYVIGREKVKKGPEKGQVCEVLKKKVDIQALRGVSLSTRQDDFFILQEDAADSFLESVFKTEFVSLLCKRFEEATRRPLPLTFSDTLQFRVKKEGWGGGGTRSVTFSRGFGDLAVLKVGGRTLTVSVGDGLPKSSKPTRKGMAKGKPRRSSQAPTRAAPAPPRGMDRNGVPPSARGGPLPLEIMSGGGTHRPPRGPPSTSLGASRRPRARPPSEHNTEFLNVPDQGMAGMQRKRSVGQRPVPGVGRPKPQPRTHGPRCRALYQYVGQDVDELSFNVNEVIEILMEDPSGWWKGRLHGQEGLFPGNYVEKI
预测分子量124,8 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于MYO1F重组蛋白的示例性参考文献(建议通过学术数据库核实具体信息):

1. **"MYO1F regulates neutrophil migration and immune response through actin remodeling"**

- **作者**: Smith A, et al.

- **摘要**: 研究揭示了MYO1F通过调控肌动蛋白动力学促进中性粒细胞迁移,重组蛋白实验表明其缺失导致免疫缺陷。

2. **"Expression and purification of functional MYO1F recombinant protein in E. coli"**

- **作者**: Chen L, et al.

- **摘要**: 开发了基于大肠杆菌的MYO1F重组表达系统,利用Ni-NTA纯化获得活性蛋白,并验证其ATP酶活性。

3. **"Structural insights into MYO1F's role in endosomal trafficking"**

- **作者**: Tanaka K, et al.

- **摘要**: 通过冷冻电镜解析MYO1F结构,发现其与内吞囊泡运输相关,重组蛋白实验支持其参与早期内体分选。

4. **"MYO1F mutations linked to pediatric immunodeficiency disorders"**

- **作者**: Rossi F, et al.

- **摘要**: 发现MYO1F基因突变与儿童免疫缺陷相关,重组蛋白功能恢复实验证实突变导致细胞迁移障碍。

**提示**:以上为模拟示例,建议使用PubMed或Google Scholar搜索“MYO1F recombinant”、“MYO1F function”等关键词获取真实文献。

背景信息

MYO1F, a member of the class I myosin family, is an unconventional myosin motor protein that links the actin cytoskeleton to cellular membranes. Unlike muscle-specific myosins, MYO1F is widely expressed in immune cells, particularly neutrophils, macrophages, and dendritic cells, where it regulates membrane dynamics, cell motility, and intracellular trafficking. It consists of a conserved N-terminal motor domain that binds actin and hydrolyzes ATP, a neck region with calmodulin-binding IQ motifs, and a C-terminal tail domain mediating membrane interactions.

This protein plays critical roles in immune responses by coordinating cytoskeletal remodeling during phagocytosis, chemotaxis, and immunological synapse formation. Studies link MYO1F dysfunction to impaired neutrophil migration, defective pathogen clearance, and inflammatory disorders. Its involvement in vesicle transport and membrane tension modulation also extends relevance to cancer metastasis and cellular mechanotransduction.

Recombinant MYO1F proteins are engineered using heterologous expression systems (e.g., baculovirus/insect or mammalian cells) to preserve post-translational modifications and functional domains. Purified MYO1F serves as a tool for in vitro actin-binding assays, ATPase activity measurements, and interaction studies with membrane phospholipids or signaling adaptors. Researchers utilize it to investigate mechanisms underlying immune deficiencies, chronic inflammation, and tumor microenvironments. Recent interest focuses on its potential as a therapeutic target for modulating immune cell behavior or inhibiting cancer cell invasion. Structural studies of recombinant MYO1F further aid in designing small-molecule inhibitors targeting its motor or lipid-binding domains.

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