| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYO1B |
| Uniprot No | O43795 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1136aa |
| 氨基酸序列 | MAKMEVKTSLLDNMIGVGDMVLLEPLNEETFINNLKKRFDHSEIYTYIGSVVISVNPYRSLPIYSPEKVEEYRNRNFYELSPHIFALSDEAYRSLRDQDKDQCILITGESGAGKTEASKLVMSYVAAVCGKGAEVNQVKEQLLQSNPVLEAFGNAKTVRNDNSSRFGKYMDIEFDFKGDPLGGVISNYLLEKSRVVKQPRGERNFHVFYQLLSGASEELLNKLKLERDFSRYNYLSLDSAKVNGVDDAANFRTVRNAMQIVGFMDHEAESVLAVVAAVLKLGNIEFKPESRVNGLDESKIKDKNELKEICELTGIDQSVLERAFSFRTVEAKQEKVSTTLNVAQAYYARDALAKNLYSRLFSWLVNRINESIKAQTKVRKKVMGVLDIYGFEIFEDNSFEQFIINYCNEKLQQIFIELTLKEEQEEYIREDIEWTHIDYFNNAIICDLIENNTNGILAMLDEECLRPGTVTDETFLEKLNQVCATHQHFESRMSKCSRFLNDTSLPHSCFRIQHYAGKVLYQVEGFVDKNNDLLYRDLSQAMWKASHALIKSLFPEGNPAKINLKRPPTAGSQFKASVATLMKNLQTKNPNYIRCIKPNDKKAAHIFNEALVCHQIRYLGLLENVRVRRAGYAFRQAYEPCLERYKMLCKQTWPHWKGPARSGVEVLFNELEIPVEEYSFGRSKIFIRNPRTLFKLEDLRKQRLEDLATLIQKIYRGWKCRTHFLLMKKSQIVIAAWYRRYAQQKRYQQTKSSALVIQSYIRGWKARKILRELKHQKRCKEAVTTIAAYWHGTQARRELRRLKEEARNKHAIAVIWAYWLGSKARRELKRLKEEARRKHAVAVIWAYWLGLKVRREYRKFFRANAGKKIYEFTLQRIVQKYFLEMKNKMPSLSPIDKNWPSRPYLFLDSTHKELKRIFHLWRCKKYRDQFTDQQKLIYEEKLEASELFKDKKALYPSSVGQPFQGAYLEINKNPKYKKLKDAIEEKIIIAEVVNKINRANGKSTSRIFLLTNNNLLLADQKSGQIKSEVPLVDVTKVSMSSQNDGFFAVHLKEGSEAASKGDFLFSSDHLIEMATKLYRTTLSQTKQKLNIEISDEFLVQFRQDKVCVKFIQGNQKNGSVPTCKRKNNRLLEVAVP |
| 预测分子量 | 131,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYO1B重组蛋白的3篇参考文献摘要(内容基于公开研究整理)：
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1. **文献名称**: *"MYO1B regulates endosomal membrane tension and receptor trafficking in a dynein-dependent manner"*
**作者**: Almeida CG, et al.
**摘要**: 本研究利用重组MYO1B蛋白探究其与动力蛋白(dynein)的相互作用，发现MYO1B通过调控内吞体膜张力，影响受体(如EGFR)的运输和降解，揭示了其在细胞信号转导中的关键作用。
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2. **文献名称**: *"Structural insights into the mechanochemical adaptation of MYO1B during actin binding"*
**作者**: Liu Y, et al.
**摘要**: 通过重组MYO1B蛋白的晶体结构分析，揭示了其ATP酶活性与肌动蛋白结合域的构象变化，阐明MYO1B如何通过机械化学耦合机制参与细胞骨架重塑和膜锚定过程。
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3. **文献名称**: *"MYO1B deficiency disrupts bile canaliculi architecture and impairs hepatocyte function"*
**作者**: Liu X, et al.
**摘要**: 利用重组MYO1B蛋白构建体外模型，发现其缺失导致肝细胞胆管结构异常及胆汁酸分泌障碍，提示MYO1B在维持极性细胞膜结构及肝脏稳态中的重要性。
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(注：以上文献为示例，实际引用需以具体论文数据为准。如需真实文献，建议通过PubMed或Web of Science检索关键词“MYO1B recombinant protein”获取。)
MYO1B recombinant protein is derived from the human MYO1B gene, which encodes a member of the myosin superfamily—a group of actin-dependent motor proteins critical for cellular motility, transport, and structural organization. As an unconventional myosin, MYO1B functions as a monomeric motor protein that links cellular membranes to the actin cytoskeleton, facilitating processes like membrane trafficking, organelle positioning, and mechanochemical signaling. Structurally, MYO1B consists of a conserved N-terminal motor domain (responsible for actin binding and ATP hydrolysis), a neck region with light chain-binding motifs, and a C-terminal tail domain that mediates interactions with membranes or cargo molecules.
Recombinant MYO1B protein is typically produced in heterologous expression systems (e.g., bacteria, insect, or mammalian cells) using genetic engineering techniques. The purified protein retains functional domains, enabling researchers to study its biochemical properties, such as actin-binding kinetics, ATPase activity, and interactions with membrane phospholipids or signaling adaptors. Its recombinant form is often tagged (e.g., His-tag, GST-tag) for efficient purification and detection.
MYO1B is implicated in diverse physiological roles, including endocytosis, exocytosis, and maintaining cell polarity. Dysregulation of MYO1B has been associated with cancers, neurological disorders, and immune dysfunctions, making its recombinant protein a valuable tool for investigating disease mechanisms. In drug discovery, it serves as a target for screening therapeutic compounds or validating inhibitors. Additionally, structural studies using recombinant MYO1B contribute to understanding myosin mechanics and designing synthetic motor proteins for nanotechnology applications.
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