| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYO1A |
| Uniprot No | Q9UBC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1043aa |
| 氨基酸序列 | MPLLEGSVGVEDLVLLEPLVEESLLKNLQLRYENKEIYTYIGNVVISVNPYQQLPIYGPEFIAKYQDYTFYELKPHIYALANVAYQSLRDRDRDQCILITGESGSGKTEASKLVMSYVAAVCGKGEQVNSVKEQLLQSNPVLEAFGNAKTIRNNNSSRFGKYMDIEFDFKGSPLGGVITNYLLEKSRLVKQLKGERNFHIFYQLLAGADEQLLKALKLERDTTGYAYLNHEVSRVDGMDDASSFRAVQSAMAVIGFSEEEIRQVLEVTSMVLKLGNVLVADEFQASGIPASGIRDGRGVREIGEMVGLNSEEVERALCSRTMETAKEKVVTALNVMQAQYARDALAKNIYSRLFDWIVNRINESIKVGIGEKKKVMGVLDIYGFEILEDNSFEQFVINYCNEKLQQVFIEMTLKEEQEEYKREGIPWTKVDYFDNGIICKLIEHNQRGILAMLDEECLRPGVVSDSTFLAKLNQLFSKHGHYESKVTQNAQRQYDHTMGLSCFRICHYAGKVTYNVTSFIDKNNDLLFRDLLQAMWKAQHPLLRSLFPEGNPKQASLKRPPTAGAQFKSSVAILMKNLYSKSPNYIRCIKPNEHQQRGQFSSDLVATQARYLGLLENVRVRRAGYAHRQGYGPFLERYRLLSRSTWPHWNGGDREGVEKVLGELSMSSGELAFGKTKIFIRSPKTLFYLEEQRRLRLQQLATLIQKIYRGWRCRTHYQLMRKSQILISSWFRGNMQKKCYGKIKASVLLIQAFVRGWKARKNYRKYFRSEAALTLADFIYKSMVQKFLLGLKNNLPSTNVLDKTWPAAPYKCLSTANQELQQLFYQWKCKRFRDQLSPKQVEILREKLCASELFKGKKASYPQSVPIPFCGDYIGLQGNPKLQKLKGGEEGPVLMAEAVKKVNRGNGKTSSRILLLTKGHVILTDTKKSQAKIVIGLDNVAGVSVTSLKDGLFSLHLSEMSSVGSKGDFLLVSEHVIELLTKMYRAVLDATQRQLTVTVTEKFSVRFKENSVAVKVVQGPAGGDNSKLRYKKKGSHCLEVTVQ |
| 预测分子量 | 118,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYO1A重组蛋白的3篇模拟参考文献示例(注：文献为虚构，仅用于示例)：
1. **文献名称**：*"Recombinant MYO1A Expression and ATPase Activity Characterization in Epithelial Cells"*
**作者**：Chen L, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组MYO1A蛋白，分析了其ATP酶活性及钙离子依赖性。结果表明，MYO1A的ATP水解活性在细胞膜运输中可能起关键作用，并与肌动蛋白结合能力相关。
2. **文献名称**：*"Structural Insights into MYO1A’s Role in Cytoskeletal Remodeling via Cryo-EM"*
**作者**：Sato K, et al.
**摘要**：利用冷冻电镜技术解析了重组MYO1A的头部结构域与肌动蛋白丝结合的分子机制，揭示了其参与细胞骨架重塑的潜在途径，为研究MYO1A在细胞极性调控中的功能提供结构基础。
3. **文献名称**：*"MYO1A Knockdown and Overexpression Models Reveal Its Function in Intestinal Barrier Integrity"*
**作者**：Müller R, et al.
**摘要**：通过构建重组MYO1A过表达/敲低细胞模型，发现MYO1A通过调控紧密连接蛋白分布维持肠道屏障完整性，其重组蛋白的体外实验进一步验证了该机制依赖其C端结构域。
4. **文献名称**：*"Interactions Between MYO1A and PIP2 in Membrane Trafficking: A Recombinant Protein Approach"*
**作者**：Wang Y, et al.
**摘要**：研究证实重组MYO1A蛋白通过其PH结构域特异性结合磷脂酰肌醇二磷酸(PIP2)，并调控内吞囊泡的运输效率，提示MYO1A在膜动力学中的脂质依赖性功能。
(注：以上文献及作者均为虚构，实际研究中请通过PubMed/Google Scholar等平台检索真实文献。)
MYO1A, a member of the myosin superfamily, is a class I myosin encoded by the MYO1A gene in humans. It belongs to a group of actin-based motor proteins that utilize ATP hydrolysis to generate mechanical force, enabling cellular processes such as membrane-cytoskeleton interactions, intracellular trafficking, and mechanotransduction. MYO1A consists of a conserved N-terminal motor domain responsible for actin binding and ATPase activity, followed by a neck region with light chain-binding motifs and a C-terminal tail domain that mediates membrane association. Unlike other myosins, class I myosins lack a coiled-coil dimerization domain, functioning as monomers.
This protein is predominantly expressed in epithelial tissues, particularly in the intestines and kidneys, where it localizes to microvilli and contributes to maintaining cell polarity and structural integrity. MYO1A facilitates the tethering of plasma membranes to the actin cytoskeleton, stabilizing dynamic cellular protrusions and regulating vesicle transport. Its role in membrane remodeling and signal transduction has implicated MYO1A in processes like nutrient absorption, cell migration, and immune responses.
Recombinant MYO1A protein is engineered for in vitro studies to dissect its molecular mechanisms, often expressed in bacterial or eukaryotic systems with affinity tags (e.g., His-tag) for purification. Researchers use it to investigate actin-binding kinetics, ATPase activity, and interactions with lipid membranes or signaling molecules. Dysregulation of MYO1A has been linked to pathologies, including colorectal cancer and hearing loss, making its recombinant form valuable for drug screening and structural analyses (e.g., cryo-EM) to design targeted therapies. Studies also explore its potential as a biomarker for epithelial-derived diseases.
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