| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIOX |
| Uniprot No | Q9UGB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-285aa |
| 氨基酸序列 | MKVTVGPDPS LVYRPDVDPE VAKDKASFRN YTSGPLLDRV FTTYKLMHTH QTVDFVRSKH AQFGGFSYKK MTVMEAVDLL DGLVDESDPD VDFPNSFHAF QTAEGIRKAH PDKDWFHLVG LLHDLGKVLA LFGEPQWAVV GDTFPVGCRP QASVVFCDST FQDNPDLQDP RYSTELGMYQ PHCGLDRVLM SWGHDEYMYQ VMKFNKFSLP PEAFYMIRFH SFYPWHTGRD YQQLCSQQDL AMLPWVREFN KFDLYTKCPD LPDVDKLRPY YQGLIDKYCP GILSW |
| 预测分子量 | 33 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MIOX重组蛋白的3-4条参考文献及其摘要概括：
1. **"Cloning, expression, and characterization of recombinant myo-inositol oxygenase from Arabidopsis thaliana"**
- **作者**: Arner, E.S.J. 等
- **摘要**: 研究报道了拟南芥MIOX基因的克隆与原核表达，纯化的重组蛋白酶动力学分析显示其对肌醇底物的高催化效率，并探讨了金属离子对其活性的调控作用。
2. **"Role of myo-inositol oxygenase in diabetic nephropathy: Insights from recombinant protein studies"**
- **作者**: Kaur, J. 等
- **摘要**: 通过体外重组MIOX蛋白实验，揭示了高血糖环境下MIOX活性上调导致氧化应激，可能与糖尿病肾病的病理进程相关。
3. **"Structural insights into the catalytic mechanism of human myo-inositol oxygenase through recombinant expression and mutagenesis"**
- **作者**: Chakraborty, A. 等
- **摘要**: 利用重组人源MIOX蛋白进行晶体结构解析和定点突变实验，阐明了其活性位点的关键氨基酸残基及底物结合机制。
4. **"Recombinant MIOX protein alleviates hepatic fibrosis via modulation of cellular redox balance"**
- **作者**: Li, X. 等
- **摘要**: 研究通过重组MIOX蛋白干预肝纤维化模型，发现其通过调节细胞氧化还原状态抑制肝星状细胞活化，为肝纤维化治疗提供潜在靶点。
(注：以上文献信息为模拟生成，实际引用时请核实真实来源。)
**Background of MIOX Recombinant Protein**
Myo-inositol oxygenase (MIOX) is a key enzyme in the *myo*-inositol degradation pathway, catalyzing the oxidation of *myo*-inositol to D-glucuronic acid. This reaction represents the rate-limiting step in the uronic acid pathway, which intersects with carbohydrate metabolism, redox regulation, and cellular osmoregulation. MIOX is predominantly expressed in mammalian kidneys and liver, where it plays a critical role in maintaining *myo*-inositol homeostasis and modulating reactive oxygen species (ROS) levels through its involvement in antioxidant pathways.
The recombinant MIOX protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems to ensure high purity and activity. Recombinant expression allows large-scale production of MIOX for functional studies, enabling researchers to explore its enzymatic mechanisms, substrate specificity, and regulatory roles in metabolic diseases. For instance, dysregulation of MIOX has been implicated in diabetic nephropathy due to its link to oxidative stress and glucose metabolism. Additionally, aberrant *myo*-inositol metabolism, mediated by MIOX, is associated with neurological disorders such as Alzheimer’s disease.
Structural studies of recombinant MIOX have revealed its dependence on divalent metal ions (e.g., Fe²⁺) for catalytic activity and provided insights into its unique substrate-binding pocket. These findings facilitate drug discovery efforts targeting MIOX-related pathologies. Furthermore, recombinant MIOX serves as a tool for developing diagnostic assays and screening inhibitors. Its role in bridging inositol metabolism with broader physiological processes underscores its biomedical relevance, making it a focal point for both basic research and therapeutic development.
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