| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTF1 |
| Uniprot No | Q01538 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 900-1101aa |
| 氨基酸序列 | GLGHISGKYASHRSASGCPLAARRQKEGSLNGSSFSWKSLKNEGPTCPTPGCDGSGHANGSFLTHRSLSGCPRATFAGKKGKLSGDEVLSPKFKTSDVLENDEEIKQLNQEIRDLNESNSEMEAAMVQLQSQISSMEKNLKNIEEENKLIEEQNEALFLELSGLSQALIQSLANIRLPHMEPICEQNFDAYVSTLTDMYSNQ |
| 预测分子量 | 26.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTF1重组蛋白的模拟参考文献示例(文献为虚构，仅作格式参考)：
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1. **文献名称**: "Expression and Functional Analysis of Recombinant MTF1 in Mammalian Cells"
**作者**: Zhang L, et al.
**摘要**: 本研究通过构建MTF1重组蛋白表达载体，在HEK293细胞中实现高效表达，并验证其在小鼠肝脏细胞中对重金属应激反应(如镉离子)的转录调控功能，证实重组MTF1可激活金属硫蛋白(MT)基因启动子。
2. **文献名称**: "Crystal Structure of Human MTF1 Zinc-Binding Domain Reveals Metal-Responsive Mechanisms"
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析MTF1锌结合结构域的重组蛋白三维结构，揭示了其与锌离子的结合模式及构象变化，为理解MTF1在重金属解毒中的分子机制提供结构基础。
3. **文献名称**: "Recombinant MTF1 Production in E. coli: Optimization and Application in DNA Binding Assays"
**作者**: Kim S, et al.
**摘要**: 优化大肠杆菌表达系统以提高MTF1重组蛋白的溶解度，利用凝胶迁移实验(EMSA)证明重组蛋白特异性结合金属响应元件(MRE)，并量化其与不同金属离子的亲和力差异。
4. **文献名称**: "MTF1 Recombinant Protein Attenuates Oxidative Stress in Zebrafish Models"
**作者**: Wang Y, et al.
**摘要**: 通过纯化MTF1重组蛋白并注射至斑马鱼胚胎，发现其通过上调抗氧化基因(如SOD、GPx)的表达显著减轻重金属诱导的氧化损伤，提示其潜在治疗应用价值。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“MTF1 recombinant protein”或“MTF1 expression and purification”获取具体研究。
**Background of MTF1 Recombinant Protein**
MTF1 (Metal Regulatory Transcription Factor 1) is a critical zinc finger transcription factor involved in cellular responses to heavy metal stress and oxidative damage. It binds to metal response elements (MREs) in promoter regions of target genes, such as metallothioneins (MTs), to regulate their expression. MTs are cysteine-rich proteins that chelate toxic metals (e.g., cadmium, mercury) and mitigate oxidative stress by scavenging free radicals. MTF1 activation is primarily triggered by elevated intracellular zinc levels, though it also responds to other metals and stressors like hypoxia or reactive oxygen species (ROS).
Structurally, MTF1 contains six zinc finger domains that mediate DNA binding and a transactivation domain essential for recruiting transcriptional machinery. Under stress, MTF1 translocates to the nucleus, binds MREs, and coordinates the upregulation of cytoprotective genes. This mechanism is vital for detoxification, metal homeostasis, and cellular survival.
Recombinant MTF1 protein is engineered for in vitro studies, often expressed in systems like *E. coli* or mammalian cells, enabling high-purity production for functional assays. Researchers use it to investigate MTF1-DNA interactions, signaling pathways, and its role in diseases linked to metal dysregulation (e.g., neurodegenerative disorders, cancer). Additionally, recombinant MTF1 aids in developing biosensors for environmental metal detection or therapeutic strategies targeting metal-associated pathologies. Its study provides insights into cellular adaptation mechanisms and potential interventions for metal toxicity or oxidative stress-related conditions.
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