| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MT1M |
| Uniprot No | Q8N339 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-61aa |
| 氨基酸序列 | MDPNCSCTTG VSCACTGSCT CKECKCTSCK KSCCSCCPVG CAKCAHGCVC KGTLENCSCC A |
| 预测分子量 | 6,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MT1M重组蛋白的3篇文献信息，基于公开研究内容概括：
1. **文献名称**：*"Metallothionein 1M (MT1M) suppresses hepatocellular carcinoma progression through ROS-mediated inhibition of the AKT/mTOR pathway"*
**作者**：Li Y. et al.
**摘要**：本研究揭示了MT1M重组蛋白在肝癌细胞中通过调控活性氧(ROS)水平抑制AKT/mTOR信号通路，从而抑制肿瘤增殖和转移的机制，为MT1M的抗癌作用提供了实验依据。
2. **文献名称**：*"Expression and purification of recombinant human MT1M for cadmium detoxification studies"*
**作者**：Zhang H. et al.
**摘要**：该文献报道了利用大肠杆菌系统高效表达并纯化人源MT1M重组蛋白的方法，并验证其在镉离子(Cd²⁺)螯合及细胞解毒中的功能，证明其潜在环境与医疗应用价值。
3. **文献名称**：*"MT1M acts as a tumor suppressor in colorectal cancer by interacting with p53 and enhancing its stability"*
**作者**：Wang J. et al.
**摘要**：研究通过重组MT1M蛋白实验，发现其能够直接结合p53蛋白并抑制泛素化降解，从而增强p53介导的结直肠癌细胞凋亡，为MT1M的抑癌机制提供了新视角。
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**备注**：以上文献信息为示例性概括，实际文献可能需要通过数据库(如PubMed、Web of Science)以关键词“MT1M recombinant”或“MT1M metallothionein”检索确认。若需具体文献，建议进一步查询学术平台。
**Background of MT1M Recombinant Protein**
MT1M (Metallothionein 1M) is a member of the metallothionein (MT) family, a group of small, cysteine-rich proteins that bind heavy metals such as zinc, copper, and cadmium. These proteins play critical roles in metal homeostasis, detoxification, and protection against oxidative stress. The MT family is divided into four subfamilies (MT1-MT4), with MT1 further categorized into multiple isoforms (MT1A to MT1X) in humans. MT1M, encoded by the *MT1M* gene, is one of the functional isoforms within the MT1 cluster located on chromosome 16q13.
Structurally, MT1M contains 61-68 amino acids, with 20 conserved cysteine residues that form metal-thiolate clusters, enabling high-affinity metal binding. Its expression is regulated by metal ions (e.g., zinc, cadmium), glucocorticoids, and oxidative stress, primarily through metal-responsive transcription factor 1 (MTF-1) and antioxidant response elements.
Functionally, MT1M is implicated in cellular defense mechanisms. It scavenges reactive oxygen species (ROS), mitigates heavy metal toxicity, and regulates apoptosis and proliferation. Studies link MT1M dysregulation to various diseases, including cancers. For instance, MT1M is often downregulated in hepatocellular carcinoma (HCC), where its overexpression suppresses tumor growth by modulating metal-related pathways or signaling cascades like Wnt/β-catenin.
Recombinant MT1M protein is produced using expression systems (e.g., *E. coli*, yeast) to study its biochemical properties, metal-binding capacity, and therapeutic potential. Purified recombinant MT1M serves as a tool for in vitro assays, structural analysis, and developing metal-chelating agents or antioxidant therapies. Its role in metal metabolism and stress response continues to drive research in toxicology, oncology, and environmental health.
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