| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MT1E |
| Uniprot No | P04732 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4-59aa |
| 氨基酸序列 | NCSCATGGSCTCAGSCKCKECKCTSCKKSCCSCCPVGCAKCAQGCVCKGASEKCSC |
| 预测分子量 | 32.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MT1E重组蛋白的3篇参考文献示例(内容为假设性概括，仅供参考)：
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1. **文献名称**：*Expression and Purification of Recombinant Human MT1E in Escherichia coli for Metal-Binding Studies*
**作者**：Smith J, Lee R, Garcia M.
**摘要**：本研究利用大肠杆菌表达系统成功克隆并纯化了重组人源MT1E蛋白，优化了诱导条件以提高产量。通过质谱和圆二色谱分析，证实重组MT1E具备与镉(Cd)、锌(Zn)等金属离子结合的能力，为后续金属解毒机制研究奠定基础。
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2. **文献名称**：*MT1E Recombinant Protein Attenuates Oxidative Stress in Human Keratinocytes*
**作者**：Zhang L, Wang H, Chen T.
**摘要**：作者通过哺乳动物细胞系表达了重组MT1E蛋白，并在人角质细胞模型中验证其抗氧化功能。实验表明，MT1E显著降低H₂O₂诱导的活性氧(ROS)水平，并通过调控Nrf2通路增强细胞对氧化损伤的抵抗。
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3. **文献名称**：*Structural Insights into the Metal Coordination Properties of MT1E via Recombinant Protein Crystallography*
**作者**：Brown K, Müller S, Ivanova A.
**摘要**：本研究通过重组MT1E蛋白的晶体结构解析，揭示了其金属结合域的关键氨基酸残基及空间构象。结果表明，MT1E对铜(Cu)的亲和力高于其他金属硫蛋白亚型，为理解其特异性生物学功能提供结构依据。
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(注：以上文献为示例，实际引用需根据具体研究检索PubMed、Web of Science等数据库获取真实信息。)
Metallothionein 1E (MT1E) is a member of the metallothionein (MT) family, a group of small, cysteine-rich proteins that bind metal ions and play essential roles in metal homeostasis, detoxification, and protection against oxidative stress. MT1E is encoded by the *MT1E* gene in humans and is part of a cluster of MT isoforms located on chromosome 16. Like other MTs, MT1E is characterized by its high cysteine content (approximately 30% of residues), which enables strong binding to divalent metal ions such as zinc (Zn²⁺), copper (Cu²⁺), and cadmium (Cd²⁺). These interactions are critical for regulating intracellular metal distribution, scavenging reactive oxygen species (ROS), and mitigating metal toxicity.
Recombinant MT1E is produced using biotechnological platforms, often through expression in bacterial (e.g., *E. coli*) or eukaryotic systems. The recombinant protein retains the native structure and metal-binding capacity, making it a valuable tool for studying metal-dependent biochemical pathways, cellular stress responses, and disease mechanisms. Research highlights its involvement in protecting cells from heavy metal poisoning, radiation damage, and oxidative injury, with potential therapeutic applications in neurodegenerative disorders, cancer, and environmental toxicity.
MT1E’s expression is induced by metal exposure, glucocorticoids, and inflammatory signals, linking it to both physiological and pathological processes. Studies also explore its role in immune modulation and apoptosis regulation. Compared to other MT isoforms, MT1E exhibits tissue-specific expression patterns and unique regulatory elements, suggesting distinct biological functions. Despite its promise, challenges remain in fully elucidating its mechanistic pathways and clinical relevance. Ongoing work aims to harness recombinant MT1E for industrial or medical use, including bioremediation, drug delivery, or as a biomarker for metal-related diseases.
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