纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | HLTF |
Uniprot No | Q14527 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-1009aa |
氨基酸序列 | MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGLRYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANNAFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQLKTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKADGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQSETKGRAKAGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWIDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMHALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKRELAAGAFGTKKPNADEMKQAKINEIRTLIDL |
预测分子量 | 113,9 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HLTF(Helicase-like Transcription Factor)重组蛋白研究的3篇示例文献(注:文献信息为模拟虚构,仅供格式参考):
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1. **文献名称**: *"Purification and Functional Characterization of Recombinant HLTF Protein in DNA Damage Repair"*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组HLTF蛋白,证实其具有ATP依赖的DNA解旋酶活性,并在体外实验中验证了HLTF在DNA损伤修复中促进同源重组的功能。
2. **文献名称**: *"Structural Insights into HLTF's Role in Replication Fork Remodeling"*
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜解析重组HLTF蛋白的三维结构,揭示了其与DNA复制叉结合的分子机制,并提出HLTF通过重塑染色质结构协助复制压力下的DNA修复。
3. **文献名称**: *"Functional Interaction between HLTF and Polycomb Repressive Complex 2"*
**作者**: Tanaka K, et al.
**摘要**: 利用重组HLTF蛋白进行体外结合实验,发现其与PRC2复合物的直接互作,表明HLTF可能通过表观遗传调控参与肿瘤抑制通路。
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如需真实文献,建议通过PubMed或Google Scholar检索关键词(如"HLTF recombinant protein" "helicase-like transcription factor purification")。
HLTF (Helicase-like Transcription Factor), also known as HIP116 or SMARCA3. is a member of the SWI/SNF chromatin remodeling protein family. It plays a dual role as both a DNA helicase and a ubiquitin ligase, contributing to genomic stability and transcriptional regulation. HLTF is primarily involved in post-replicative DNA repair pathways, particularly in error-free DNA damage tolerance mechanisms such as template switch repair. Its conserved helicase domain enables ATP-dependent chromatin remodeling, while the RING finger domain facilitates polyubiquitination of proliferating cell nuclear antigen (PCNA), a key regulator of DNA replication forks.
Recombinant HLTF proteins are engineered for functional studies due to challenges in isolating native forms from biological systems. These proteins are typically expressed in *E. coli* or mammalian cell lines using plasmid vectors, often fused with tags (e.g., His-tag, GST) for purification via affinity chromatography. Researchers utilize recombinant HLTF to investigate its structure-function relationships, enzymatic activities, and interactions with DNA repair complexes. Notably, HLTF shows paradoxical roles in cancer biology – acting as a tumor suppressor through DNA repair functions in some contexts, while displaying oncogenic potential via epigenetic dysregulation in others. Its involvement in homologous recombination repair has sparked interest in synthetic lethality strategies for cancers with defective DNA repair pathways. Current studies focus on characterizing mutations, post-translational modifications, and small-molecule inhibitors targeting HLTF's enzymatic domains for therapeutic development.
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