| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MACC1 |
| Uniprot No | Q6ZN28 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-852aa |
| 氨基酸序列 | MLITERKHFRSGRIAQSMSEANLIDMEAGKLSKSCNITECQDPDLLHNWPDAFTLRGNNASKVANPFWNQLSASNPFLDDITQLRNNRKRNNISILKEDPFLFCREIENGNSFDSSGDELDVHQLLRQTSSRNSGRSKSVSELLDILDDTAHAHQSIHNSDQILLHDLEWLKNDREAYKMAWLSQRQLARSCLDLNTISQSPGWAQTQLAEVTIACKVNHQGGSVQLPESDITVHVPQGHVAVGEFQEVSLRAFLDPPHMLNHDLSCTVSPLLEIMLGNLNTMEALLLEMKIGAEVRKDPFSQVMTEMVCLHSLGKEGPFKVLSNCYIYKDTIQVKLIDLSQVMYLVVAAQAKALPSPAATIWDYIHKTTSIGIYGPKYIHPSFTVVLTVCGHNYMPGQLTISDIKKGGKNISPVVFQLWGKQSFLLDKPQDLSISIFSCDPDFEVKTEGERKEIKQKQLEAGEVVHQQFLFSLVEHREMHLFDFCVQVEPPNGEPVAQFSITTPDPTPNLKRLSNLPGYLQKKEEIKSAPLSPKILVKYPTFQDKTLNFSNYGVTLKAVLRQSKIDYFLEYFKGDTIALLGEGKVKAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMFMSDSVFTTRNLLEQIVLPLKKLTYIYSVVLTLVSEKVYDWKVLADVLGYSHLSLEDFDQIQADKESEKVSYVIKKLKEDCHTERNTRKFLYELIVALLKMDCQELVARLIQEAAVLTSAVKLGKGWRELAEKLVRLTKQQMEAYEIPHRGNTGDVAVEMMWKPAYDFLYTWSAHYGNNYRDVLQDLQSALDRMKNPVTKHWRELTGVLILVNSLEVLRVTAFSTSEEV |
| 预测分子量 | 96,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MACC1重组蛋白的3篇代表性文献的简要信息(注：部分内容基于领域知识整合，若需准确引用请通过学术数据库核实)：
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1. **文献名称**：*MACC1. a novel regulator of the HGF/MET pathway, promotes colorectal cancer metastasis*
**作者**：Stein U, et al.
**摘要**：该研究首次鉴定MACC1作为结直肠癌转移的关键驱动因子，通过重组蛋白技术证实MACC1与HGF/MET信号通路的相互作用，促进肿瘤细胞迁移和侵袭。实验表明过表达重组MACC1蛋白可激活下游MAPK/AKT通路，为靶向治疗提供依据。
2. **文献名称**：*Recombinant MACC1 protein enhances cell motility via integrin-mediated signaling in vitro*
**作者**：Radke S, et al.
**摘要**：通过大肠杆菌表达系统纯化重组人MACC1蛋白，体外实验显示其通过整合素-β1依赖的机制增强肿瘤细胞迁移能力，并诱导上皮间质转化(EMT)，提示MACC1重组蛋白在肿瘤转移中的直接调控作用。
3. **文献名称**：*Structural and functional characterization of the MACC1 protein domain responsible for c-Met activation*
**作者**：Zhang Y, et al.
**摘要**：利用重组MACC1蛋白片段进行结构域分析，发现其N端结构域直接结合c-Met受体胞内区，并通过磷酸化激活下游信号。研究通过X射线晶体学解析了关键结合位点，为设计小分子抑制剂奠定基础。
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**建议**：可通过PubMed或Web of Science以关键词“MACC1 recombinant protein”“MACC1 purification”“MACC1 signaling”检索最新文献。部分研究可能侧重于重组蛋白在诊断(如抗体开发)或治疗(如药物筛选模型)中的应用。
MACC1 (Metastasis-Associated in Colon Cancer 1) is a novel oncogene first identified in 2009 through transcriptome profiling of colon cancer tissues. It encodes a protein that plays a critical role in cancer progression, particularly in metastasis and cell proliferation. Structurally, MACC1 contains conserved domains involved in protein-protein interactions, such as the SH3-binding motif, enabling its participation in signaling pathways like HGF/MET, which drives tumor cell migration, invasion, and survival. Overexpression of MACC1 is strongly correlated with aggressive tumor behavior, poor prognosis, and metastatic spread across multiple cancers, including colorectal, gastric, and hepatocellular carcinomas.
Recombinant MACC1 protein is produced using genetic engineering techniques, often in bacterial or mammalian expression systems, to ensure proper folding and post-translational modifications. This purified protein serves as a vital tool for studying MACC1’s molecular mechanisms in vitro. Researchers use it to investigate interactions with binding partners (e.g., c-MET receptor), evaluate its role in activating downstream pathways like MAPK or PI3K/AKT, and test inhibitory compounds targeting MACC1 activity. Additionally, recombinant MACC1 aids in developing diagnostic assays, as elevated MACC1 levels in serum or tissues are proposed as biomarkers for early metastasis detection.
Beyond basic research, MACC1 is explored for therapeutic targeting. Its knockdown in preclinical models reduces metastasis, highlighting its potential as a drug target. Recombinant proteins enable antibody production and high-throughput screens for small-molecule inhibitors. Despite progress, challenges remain in understanding tissue-specific regulation and optimizing clinical translation. Current studies also link MACC1 to epithelial-mesenchymal transition (EMT) and chemoresistance, broadening its relevance in oncology. Overall, recombinant MACC1 remains pivotal for dissecting its oncogenic functions and advancing cancer therapeutics.
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