| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRB1 |
| Uniprot No | P04280 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 17-103aa |
| 氨基酸序列 | QNLNEDVSQEESPSLIAGNPQGPSPQGGNKPQGPPPPPGKPQGPPPQGGNKPQGPPPPGKPQGPPPQGDKSRSPRSPPGKPQGPPPQ |
| 预测分子量 | 40.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRB1重组蛋白的3篇示例参考文献(内容为模拟虚构，供参考)：
1. **文献名称**: *"Cloning, expression, and functional characterization of recombinant PRB1 in a prokaryotic system"*
**作者**: Smith A, et al.
**摘要**: 本研究报道了通过PCR扩增获得人类PRB1基因编码序列，并在大肠杆菌中成功表达重组PRB1蛋白。纯化后的蛋白经Western blot验证，并证实其在体外具有调控细胞黏附和迁移的生物学功能。
2. **文献名称**: *"Structural analysis of PRB1 recombinant protein and its interaction with collagen"*
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了重组PRB1蛋白的三维结构，揭示了其N端结构域与胶原蛋白结合的分子机制，为开发基于PRB1的靶向治疗策略提供了结构基础。
3. **文献名称**: *"Recombinant PRB1 attenuates inflammatory response in a murine model of colitis"*
**作者**: Chen L, et al.
**摘要**: 在小鼠结肠炎模型中，外源性重组PRB1蛋白通过抑制NF-κB信号通路显著减轻肠道炎症反应，提示PRB1在免疫调节中的潜在治疗价值。
注：以上文献信息为示例，实际研究中建议通过PubMed或Web of Science等数据库检索真实文献。
**Background of PRB1 Recombinant Protein**
PRB1 (Proline-Rich Protein BstNI Subfamily 1) is a member of the proline-rich protein family, primarily expressed in salivary glands and secreted into the oral cavity. These proteins play critical roles in maintaining oral health by contributing to lubrication, enamel mineralization, and microbial defense. PRB1. characterized by its high proline and glycine content, interacts with oral microbes and may influence bacterial adhesion, biofilm formation, and tooth surface protection. Dysregulation of PRB1 has been linked to oral diseases, including dental caries and periodontal disorders.
Recombinant PRB1 protein is engineered using genetic cloning techniques, where the PRB1 gene is inserted into expression vectors (e.g., bacterial, yeast, or mammalian systems) to produce purified protein for research. This approach allows controlled study of PRB1’s structural and functional properties, bypassing challenges in isolating it from natural sources. Recombinant PRB1 retains biological activity, enabling investigations into its binding partners, antimicrobial effects, and role in modulating immune responses in oral tissues.
Studies utilizing recombinant PRB1 focus on its potential therapeutic applications, such as designing antimicrobial peptides or biomimetic materials for dental care. It also serves as a tool to explore saliva-based diagnostics for oral/systemic diseases. Research on PRB1 contributes to understanding the molecular basis of oral homeostasis and informs strategies for preventing or treating microbiome-related disorders.
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