| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRE11A |
| Uniprot No | P49959 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-708aa |
| 氨基酸序列 | STADALDDE NTFKILVATD IHLGFMEKDA VRGNDTFVTL DEILRLAQEN EVDFILLGGD LFHENKPSRK TLHTCLELLR KYCMGDRPVQ FEILSDQSVN FGFSKFPWVN YQDGNLNISI PVFSIHGNHD DPTGADALCA LDILSCAGFV NHFGRSMSVE KIDISPVLLQ KGSTKIALYG LGSIPDERLY RMFVNKKVTM LRPKEDENSW FNLFVIHQNR SKHGSTNFIP EQFLDDFIDL VIWGHEHECK IAPTKNEQQL FYISQPGSSV VTSLSPGEAV KKHVGLLRIK GRKMNMHKIP LHTVRQFFME DIVLANHPDI FNPDNPKVTQ AIQSFCLEKI EEMLENAERE RLGNSHQPEK PLVRLRVDYS GGFEPFSVLR FSQKFVDRVA NPKDIIHFFR HREQKEKTGE EINFGKLITK PSEGTTLRVE DLVKQYFQTA EKNVQLSLLT ERGMGEAVQE FVDKEEKDAI EELVKYQLEK TQRFLKERHI DALEDKIDEE VRRFRETRQK NTNEEDDEVR EAMTRARALR SQSEESASAF SADDLMSIDL AEQMANDSDD SISAATNKGR GRGRGRRGGR GQNSASRGGS QRGRADTGLE TSTRSRNSKT AVSASRNMSI IDAFKSTRQQ PSRNVTTKNY SEVIEVDESD VEEDIFPTTS KTDQRWSSTS SSKIMSQSQV SKGVDFESSE DDDDDPFMNT SSLRRNRR |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRE11A重组蛋白的3篇代表性文献，内容涵盖其功能、结构及作用机制：
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1. **"The Mre11:Rad50 structure: An integrated approach to substrate recognition and nuclease regulation"**
- **作者**: Williams, G.J. 等
- **摘要**: 该研究通过重组表达纯化MRE11A和RAD50蛋白，解析了二者复合物的晶体结构，揭示了MRE11A核酸酶活性对DNA末端识别的分子机制，并阐明了RAD50如何通过ATP依赖的构象变化调控MRE11A的酶活性。
2. **"Mre11 dimers coordinate DNA end bridging and nuclease processing in double-strand-break repair"**
- **作者**: Deshpande, R.A. 等
- **摘要**: 利用重组MRE11A蛋白进行体外生化实验，发现MRE11A以二聚体形式结合DNA断裂末端，通过协同核酸内切酶和外切酶活性加工DNA，为同源重组修复(HRR)提供必要的单链DNA区域。
3. **"Functional characterization of the Mre11-Rad50-Nbs1 complex in DNA damage repair using reconstituted recombinant proteins"**
- **作者**: Lee, J.H. 等
- **摘要**: 研究通过共表达和纯化MRE11A、RAD50及NBS1重组蛋白，重建了完整的MRN复合体，验证了其在DNA损伤信号传导(如ATM激酶激活)及末端切除中的关键作用，并揭示了其与肿瘤抑制蛋白p53的相互作用。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对具体信息。研究多聚焦于MRE11A的酶活性调控、复合物组装及其在DNA修复中的核心功能。
The MRE11A protein, encoded by the *MRE11* gene in humans, is a critical component of the MRE11-RAD50-NBS1 (MRN) complex, which plays a central role in DNA damage response and repair mechanisms. MRE11A exhibits endo- and exonuclease activities, enabling it to process DNA double-strand breaks (DSBs) by resecting damaged ends, a prerequisite for homologous recombination repair (HRR) and non-homologous end joining (NHEJ). Its interaction with RAD50 and NBS1 enhances its enzymatic activity and facilitates signal transduction to activate cell cycle checkpoints, allowing cells to pause division and repair DNA lesions.
Recombinant MRE11A proteins are engineered in vitro using expression systems like *E. coli*, insect, or mammalian cells, often fused with tags (e.g., His-tag) for purification and detection. These proteins are indispensable tools for studying DSB repair mechanisms, protein-protein interactions, and enzymatic kinetics in controlled environments. Research leveraging recombinant MRE11A has revealed insights into its structure-function relationships, including how mutations (e.g., hypomorphic variants linked to ataxia-telangiectasia-like disorder, ATLD) impair nuclease activity or disrupt MRN complex formation.
Clinically, MRE11A dysfunction is associated with genomic instability, cancer predisposition, and radiation sensitivity. Recombinant forms are also used to screen therapeutic agents targeting DNA repair pathways, particularly in cancers with HRR deficiencies (e.g., BRCA-mutated tumors). Despite its importance, unresolved questions remain about its regulation and context-specific roles in replication stress or telomere maintenance. Ongoing studies aim to refine its therapeutic targeting while balancing efficacy and toxicity in clinical applications.
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