| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ALK |
| Uniprot No | Q9UM73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1060-1620aa |
| 氨基酸序列 | RRKHQELQAMQMELQSPEYKLSKLRTSTIMTDYNPNYCFAGKTSSISDLK EVPRKNITLIRGLGHGAFGEVYEGQVSGMPNDPSPLQVAVKTLPEVCSEQ DELDFLMEALIISKFNHQNIVRCIGVSLQSLPRFILLELMAGGDLKSFLR ETRPRPSQPSSLAMLDLLHVARDIACGCQYLEENHFIHRDIAARNCLLTC PGPGRVAKIGDFGMARDIYRASYYRKGGCAMLPVKWMPPEAFMEGIFTSK TDTWSFGVLLWEIFSLGYMPYPSKSNQEVLEFVTSGGRMDPPKNCPGPVY RIMTQCWQHQPEDRPNFAIILERIEYCTQDPDVINTALPIEYGPLVEEEE KVPVRPKDPEGVPPLLVSQQAKREEERSPAAPPPLPTTSSGKAAKKPTAA EISVRVPRGPAVEGGHVNMAFSQSNPPSELHKVHGSRNKPTSLWNPTYGS WFTEKPTKKNNPIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEP SSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTIL KSKNSMNQPGP |
| 预测分子量 | 90 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ALK重组蛋白的3-4篇代表性文献，按文献名称、作者和摘要内容简要列举：
1. **文献名称**：*Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer*
**作者**：Soda, M., et al. (2007)
**摘要**：首次报道了EML4-ALK融合基因在非小细胞肺癌中的致癌作用，阐明了ALK基因重排导致组成性激酶活化的机制。
2. **文献名称**：*Structural analysis of the autoinhibited kinase domain of ALK using recombinant protein expression*
**作者**：Bresler, S.C., et al. (2011)
**摘要**：通过重组ALK激酶结构域的蛋白表达与晶体学研究，揭示了ALK的自抑制构象及致癌突变对激酶活化的影响。
3. **文献名称**：*Targeting ALK in neuroblastoma: preclinical and clinical advancements*
**作者**：Shaw, A.T., Solomon, B. (2011)
**摘要**：综述ALK基因在神经母细胞瘤中的重组突变及其作为治疗靶点的潜力，强调重组ALK蛋白在药物筛选中的关键作用。
4. **文献名称**：*Alectinib versus crizotinib in ALK-positive non-small-cell lung cancer*
**作者**：Friboulet, L., et al. (2014)
**摘要**：基于重组ALK蛋白的体外实验和临床研究，对比二代ALK抑制剂(艾乐替尼)与克唑替尼的疗效及耐药机制。
以上文献覆盖了ALK重组蛋白在致癌机制、结构解析、靶向治疗及临床应用中的核心研究。
Anaplastic lymphoma kinase (ALK) is a receptor tyrosine kinase first identified in 1994 through its involvement in chromosomal rearrangements associated with anaplastic large cell lymphoma (ALCL). The ALK gene encodes a transmembrane protein comprising an extracellular ligand-binding domain, a transmembrane segment, and an intracellular tyrosine kinase domain. Under normal physiological conditions, ALK plays roles in nervous system development, but its expression in adults is largely restricted to specific tissues.
ALK gained prominence in oncology due to its oncogenic potential when altered. Chromosomal rearrangements, such as the NPM-ALK fusion in ALCL or EML4-ALK in non-small cell lung cancer (NSCLC), result in constitutive kinase activation, driving uncontrolled cell proliferation and survival. These discoveries established ALK as a critical therapeutic target, leading to the development of tyrosine kinase inhibitors (TKIs) like crizotinib and alectinib.
Recombinant ALK proteins are engineered to study ALK biology and drug interactions. Produced via expression systems (e.g., bacterial, insect, or mammalian cells), these proteins retain functional domains required for ligand binding or kinase activity. They serve as tools for structural studies, inhibitor screening, and antibody development. For example, recombinant ALK kinase domain proteins enable high-throughput drug testing, while extracellular domains aid in characterizing antibody-based therapies.
In research, ALK recombinant proteins help dissect signaling pathways and mutation effects (e.g., resistance mutations like L1196M). Clinically, they support diagnostic assays to detect ALK rearrangements in tumors and validate therapeutic efficacy. Despite advancements, challenges remain in understanding ALK's full interactome and optimizing inhibitors to overcome resistance. Continued use of recombinant ALK proteins remains pivotal for both basic research and translational applications in precision oncology.
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