| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLK12 |
| Uniprot No | Q9UKR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-248aa |
| 氨基酸序列 | ATPKIFNGTECGRNSQPWQVGLFEGTSLRCGGVLIDHRWVLTAAHCSGSRYWVRLGEHSLSQLDWTEQIRHSGFSVTHPGYLGASTSHEHDLRLLRLRLPVRVTSSVQPLPLPNDCATAGTECHVSGWGITNHPRNPFPDLLQCLNLSIVSHATCHGVYPGRITSNMVCAGGVPGQDACQGDSGGPLVCGGVLQGLVSWGSVGPCGQDGIPGVYTYICKYVDWIRMIMRNN |
| 预测分子量 | 32.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLK12重组蛋白的模拟参考文献示例(请注意，文献信息为假设，实际引用需核实真实来源)：
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1. **标题**: *Expression and Functional Characterization of Recombinant Human Kallikrein 12 in a Mammalian Cell System*
**作者**: Müller, S. et al.
**摘要**: 研究通过哺乳动物细胞系统成功表达并纯化重组KLK12蛋白，证实其具有胰蛋白酶样活性，并可特异性水解纤连蛋白，提示其在细胞外基质重塑中的潜在作用。
2. **标题**: *KLK12 Recombinant Protein as a Novel Biomarker for Colorectal Cancer: Enzymatic Activity and Clinical Correlation*
**作者**: Tanaka, K. et al.
**摘要**: 分析重组KLK12在结直肠癌组织中的表达水平，发现其酶活性与肿瘤转移呈正相关，或可作为预后评估的生物标志物。
3. **标题**: *Structural and Biochemical Insights into the Substrate Specificity of Kallikrein 12 via Recombinant Protein Mutagenesis*
**作者**: Zhang, L. et al.
**摘要**: 通过定点突变重组KLK12.揭示其底物结合口袋的关键氨基酸残基，为设计选择性抑制剂提供结构基础。
4. **标题**: *Recombinant KLK12 Modulates Inflammatory Responses in Keratinocytes: Implications for Psoriasis Pathogenesis*
**作者**: Rossi, E. et al.
**摘要**: 利用重组KLK12处理人角质形成细胞，发现其通过激活PAR-2受体加剧炎症因子释放，可能参与银屑病的发展机制。
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**注意**：以上内容为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议使用关键词“KLK12 recombinant protein”“kallikrein 12 expression”进行精确查询。
**Background of KLK12 Recombinant Protein**
Kallikrein-related peptidase 12 (KLK12) is a serine protease belonging to the human tissue kallikrein (KLK) family, which comprises 15 secreted enzymes (KLK1-KLK15) encoded by a gene cluster on chromosome 19. KLKs are involved in diverse physiological and pathological processes, including tissue remodeling, inflammation, and cancer progression. KLK12. first identified in 2001. is expressed in various tissues, such as the brain, skin, and reproductive organs, though its physiological substrates and precise biological roles remain less characterized compared to other KLKs.
Structurally, KLK12 shares conserved features with other KLKs, including a catalytic triad (His, Asp, Ser) and a preference for cleaving peptide bonds after arginine or lysine residues. Its activity is regulated by endogenous inhibitors (e.g., serpins) and pH-dependent zymogen activation. Emerging evidence suggests KLK12 may participate in skin barrier function, neuronal signaling, and tumor microenvironments. Dysregulated KLK12 expression has been observed in cancers (e.g., ovarian, lung) and inflammatory disorders, hinting at its potential as a diagnostic biomarker or therapeutic target.
Recombinant KLK12 is produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its enzymatic properties, substrate specificity, and interactions. Purification often involves affinity chromatography followed by refolding or activation steps to ensure functional activity. Researchers utilize KLK12 recombinant protein in *in vitro* assays (e.g., fluorogenic substrate hydrolysis), cell-based studies, and animal models to elucidate its roles in disease pathways or normal physiology.
Despite progress, KLK12's full mechanistic landscape remains under investigation, driving demand for high-quality recombinant tools to advance its functional characterization and translational applications.
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