| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LIPE |
| Uniprot No | Q05469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1076aa |
| 氨基酸序列 | MEPGSKSVSRSDWQPEPHQRPITPLEPGPEKTPIAQPESKTLQGSNTQQKPASNQRPLTQQETPAQHDAESQKEPRAQQKSASQEEFLAPQKPAPQQSPYIQRVLLTQQEAASQQGPGLGKESITQQEPALRQRHVAQPGPGPGEPPPAQQEAESTPAAQAKPGAKREPSAPTESTSQETPEQSDKQTTPVQGAKSKQGSLTELGFLTKLQELSIQRSALEWKALSEWVTDSESESDVGSSSDTDSPATMGGMVAQGVKLGFKGKSGYKVMSGYSGTSPHEKTSARNHRHYQDTASRLIHNMDLRTMTQSLVTLAEDNIAFFSSQGPGETAQRLSGVFAGVREQALGLEPALGRLLGVAHLFDLDPETPANGYRSLVHTARCCLAHLLHKSRYVASNRRSIFFRTSHNLAELEAYLAALTQLRALVYYAQRLLVTNRPGVLFFEGDEGLTADFLREYVTLHKGCFYGRCLGFQFTPAIRPFLQTISIGLVSFGEHYKRNETGLSVAASSLFTSGRFAIDPELRGAEFERITQNLDVHFWKAFWNITEMEVLSSLANMASATVRVSRLLSLPPEAFEMPLTADPTLTVTISPPLAHTGPGPVLVRLISYDLREGQDSEELSSLIKSNGQRSLELWPRPQQAPRSRSLIVHFHGGGFVAQTSRSHEPYLKSWAQELGAPIISIDYSLAPEAPFPRALEECFFAYCWAIKHCALLGSTGERICLAGDSAGGNLCFTVALRAAAYGVRVPDGIMAAYPATMLQPAASPSRLLSLMDPLLPLSVLSKCVSAYAGAKTEDHSNSDQKALGMMGLVRRDTALLLRDFRLGASSWLNSFLELSGRKSQKMSEPIAEPMRRSVSEAALAQPQGPLGTDSLKNLTLRDLSLRGNSETSSDTPEMSLSAETLSPSTPSDVNFLLPPEDAGEEAEAKNELSPMDRGLGVRAAFPEGFHPRRSSQGATQMPLYSSPIVKNPFMSPLLAPDSMLKSLPPVHIVACALDPMLDDSVMLARRLRNLGQPVTLRVVEDLPHGFLTLAALCRETRQAAELCVERIRLVLTPPAGAGPSGETGAAGVDGGCGGRH |
| 预测分子量 | 116,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LIPE重组蛋白的示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库检索确认)：
1. **文献名称**: "Cloning and Functional Characterization of Recombinant Human LIPE/Hormone-Sensitive Lipase"
**作者**: Smith J, et al.
**摘要**: 本研究报道了人源LIPE基因的克隆及其在大肠杆菌中的重组表达，验证了重组蛋白的脂解活性及其在脂肪代谢调控中的作用。
2. **文献名称**: "Expression and Purification of Active LIPE in Mammalian Cells for Adipocyte Signaling Studies"
**作者**: Lee C, et al.
**摘要**: 通过哺乳动物细胞系统成功表达并纯化功能性LIPE重组蛋白，证实其参与cAMP-PKA信号通路，并影响脂肪细胞分化过程。
3. **文献名称**: "Structural Insights into LIPE Activation Mechanisms via Recombinant Protein Crystallography"
**作者**: Zhang R, et al.
**摘要**: 利用重组LIPE蛋白的晶体结构解析，揭示了其磷酸化激活的分子机制，为靶向LIPE的代谢疾病治疗提供结构基础。
4. **文献名称**: "Optimization of LIPE Recombinant Production in Yeast for High-Throughput Drug Screening"
**作者**: Garcia M, et al.
**摘要**: 开发了一种酵母表达系统高效生产LIPE重组蛋白的方法，并应用于小分子抑制剂筛选，发现潜在抗肥胖化合物。
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建议通过PubMed、Google Scholar等平台以关键词 **"LIPE recombinant protein"** 或 **"hormone-sensitive lipase expression"** 检索最新文献，优先选择高被引或近5年发表的研究。
**Background of LIPE Recombinant Protein**
LIPE (Lipase E, hormone-sensitive type), also known as hormone-sensitive lipase (HSL), is a key enzyme encoded by the *LIPE* gene in humans. It plays a central role in lipid metabolism, particularly in the hydrolysis of stored triglycerides (TGs) into free fatty acids (FFAs) and glycerol, a process critical for energy mobilization in adipose tissue. HSL is activated in response to hormonal signals, such as adrenaline and noradrenaline via β-adrenergic receptors, and is inhibited by insulin. Beyond adipose tissue, LIPE is expressed in steroidogenic tissues (e.g., adrenal glands, ovaries, testes), where it regulates cholesterol ester hydrolysis to support steroid hormone synthesis. Dysregulation of LIPE activity is linked to metabolic disorders, including obesity, type 2 diabetes, and cardiovascular diseases.
Recombinant LIPE protein is produced using biotechnological methods, often in prokaryotic (e.g., *E. coli*) or eukaryotic (e.g., mammalian, insect cell) expression systems. This engineered protein retains the functional domains of native HSL, including the catalytic domain responsible for lipase activity and regulatory regions that mediate phosphorylation-dependent activation (e.g., Ser-563 and Ser-565 in humans). Purification techniques, such as affinity chromatography, ensure high purity and bioactivity.
Research applications of recombinant LIPE include studying its enzymatic kinetics, interaction with regulatory proteins (e.g., perilipin), and role in metabolic pathways. It is also used to screen potential therapeutics targeting obesity or insulin resistance. Additionally, LIPE’s involvement in steroidogenesis makes it relevant in endocrine research. Challenges in working with recombinant LIPE include maintaining its stability and phosphorylation-dependent activity *in vitro*. Overall, recombinant LIPE serves as a vital tool for unraveling lipid metabolism mechanisms and developing treatments for metabolic diseases.
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