| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKAP11 |
| Uniprot No | Q9UKA4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AKAP11重组蛋白的3篇参考文献示例：
1. **"AKAP11 regulates Wnt/β-catenin signaling by targeting dishevelled to the microtubules"**
- **作者**: Smith J, et al.
- **摘要**: 研究通过重组AKAP11蛋白揭示了其与Dvl(Dishevelled)蛋白的相互作用，证明AKAP11通过将Dvl锚定至微管调控Wnt/β-catenin信号通路，影响细胞极性和肿瘤发生。
2. **"Molecular cloning and functional analysis of AKAP11 in spermatogenesis"**
- **作者**: Chen L, et al.
- **摘要**: 报道了AKAP11重组蛋白的克隆与表达，发现其通过结合PKA(蛋白激酶A)调控精子形成过程中的微管动力学，缺失导致雄性小鼠不育。
3. **"AKAP11 genetic variation and its interaction with DISC1 in psychiatric disorders"**
- **作者**: Wang Y, et al.
- **摘要**: 利用重组AKAP11蛋白进行体外结合实验，证实其与精神疾病相关蛋白DISC1的相互作用，提示AKAP11基因突变可能通过破坏信号复合物参与精神分裂症发病机制。
注：以上文献为示例，实际引用需根据具体研究内容查询真实数据库(如PubMed、Web of Science)。
The A-kinase anchoring protein 11 (AKAP11) is a member of the AKAP family, which functions as scaffolding proteins to compartmentalize cyclic AMP (cAMP)-dependent protein kinase A (PKA) and other signaling molecules within specific subcellular regions. By anchoring PKA and associated regulators, AKAP11 facilitates localized phosphorylation events, ensuring precise spatiotemporal control of cellular signaling pathways. It plays roles in diverse processes, including cell cycle regulation, cytoskeletal organization, and metabolic homeostasis. Dysregulation of AKAP11 has been implicated in diseases such as cancer, neurodegenerative disorders, and polycystic kidney disease, highlighting its physiological and pathological significance.
Recombinant AKAP11 protein is engineered using heterologous expression systems (e.g., bacterial, mammalian, or insect cells) to produce purified, functional protein for experimental applications. This involves cloning the AKAP11 gene into expression vectors, optimizing conditions for high yield, and employing affinity tags (e.g., His-tag) for efficient purification. Recombinant AKAP11 retains key structural domains, such as PKA-binding motifs and regions mediating interactions with other signaling components, enabling studies on its molecular mechanisms.
Researchers utilize recombinant AKAP11 to investigate its role in organizing signalosomes, map binding interfaces with partners like PKA or β-catenin, and explore its involvement in disease pathways. It is also valuable for structural studies, drug screening, and developing therapeutic strategies targeting AKAP11-associated disorders. By providing a controlled, scalable source of the protein, recombinant AKAP11 advances both basic and translational research in cellular signaling and disease biology.
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