| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASCC3 |
| Uniprot No | Q8N3C0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-111aa |
| 氨基酸序列 | MALPRLTGALRSFSNVTKQDNYNEEVADLKIKRSKLHEQVLDLGLTWKKIIKFLNEKLEKSKMQSINEDLKDILHAAKQIEVNCPFQKRRLDGKEEDEKMSRASDRFRGLR |
| 预测分子量 | 40.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASCC3重组蛋白的3篇参考文献的简要概括：
1. **文献名称**: *Structural insights into the ASCC3 subunit of the ASCC complex*
**作者**: Smith J, et al.
**摘要**: 该研究利用重组表达的ASCC3蛋白解析其晶体结构，揭示了其C端螺旋结构域在DNA损伤修复中的关键作用，并证明其与RNA解旋酶活性相关。
2. **文献名称**: *Functional characterization of recombinant ASCC3 in transcriptional regulation*
**作者**: Lee S, Kim D.
**摘要**: 通过体外重组ASCC3蛋白实验，发现其与RNA聚合酶II相互作用，调控靶基因转录，并验证其SUMO化修饰对功能的影响。
3. **文献名称**: *ASCC3 recombinase activity in alkylation damage repair*
**作者**: Müller C, et al.
**摘要**: 研究证实重组ASCC3蛋白具有位点特异性DNA重组酶活性，可识别烷基化损伤位点，协同ALKBH3促进DNA修复通路。
(注：以上文献信息为示例模板，实际引用时需根据具体文献调整内容。建议通过PubMed或Web of Science以"ASCC3 recombinant"为关键词检索最新研究。)
ASCC3 (Activating Signal Cointegrator Complex Subunit 3) is a helicase protein encoded by the ASCC3 gene, playing critical roles in nucleic acid metabolism and genome maintenance. As part of the ASC-1 complex, it participates in transcriptional regulation and DNA damage response pathways. Structurally, ASCC3 contains conserved DEXH-box helicase domains, enabling ATP-dependent unwinding of DNA-RNA hybrids (R-loops) and resolving transcription-replication conflicts, thereby safeguarding genomic stability. Dysregulation of ASCC3 is implicated in cancer, neurodegenerative disorders, and aging-related pathologies due to its role in mitigating replication stress and preventing R-loop accumulation.
Recombinant ASCC3 proteins are engineered in vitro using expression systems like Escherichia coli or mammalian cells, often fused with tags (e.g., GST, His) for purification and detection. These proteins retain enzymatic activity and are indispensable for studying helicase mechanisms, protein-DNA/RNA interactions, and cellular responses to genotoxic agents. Researchers utilize recombinant ASCC3 to screen helicase inhibitors, model disease-associated mutations, and explore therapeutic strategies targeting DNA repair pathways. Its application extends to structural studies (e.g., cryo-EM) to resolve conformational dynamics during substrate processing. As a tool, recombinant ASCC3 bridges molecular biology and translational research, offering insights into genome integrity regulation and potential drug development avenues.
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