| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRMP1 |
| Uniprot No | Q14194 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-572aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSEFMSYQGKKSIPHITSDRLLIKGGRII NDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTY LQKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHE AADTKSCCDYSLHVDITSWYDGVREELEVLVQDKGVNSFQVYMAYKDVYQ MSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMGITGPEGHAL SRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVF GEPIAASLGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGD LQVTGSGHCPYSTAQKAVGKDNFTLIPEGVNGIEERMTVVWDKAVATGKM DENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSH KSAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFP EHLYQRVKIRNKVFGLQGVSRGMYDGPVYEVPATPKYATPAPSAKSSPSK HQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPPGGRSNITSLG |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRMP1重组蛋白的参考文献示例(注：文献为假设性示例，仅供参考)：
---
1. **文献名称**: *"Recombinant CRMP1 Expression in E. coli and Its Role in Neurite Outgrowth"*
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌表达系统成功制备了重组CRMP1蛋白，并证明其在体外神经元培养中促进神经突生长，提示CRMP1在神经发育中的潜在作用。
2. **文献名称**: *"Structural Insights into CRMP1-Tubulin Interaction Using Recombinant Protein Crystallography"*
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析重组CRMP1的三维结构，揭示其与微管蛋白结合的特定结构域，为CRMP1调控微管组装的分子机制提供证据。
3. **文献名称**: *"CRMP1 Recombinant Protein Modulates Dendritic Spine Plasticity via RhoA Signaling"*
**作者**: Tanaka K, et al.
**摘要**: 研究发现，重组CRMP1蛋白通过调控RhoA信号通路影响海马神经元树突棘可塑性，表明其在突触功能中的关键作用。
4. **文献名称**: *"Phosphorylation-Specific Effects of Recombinant CRMP1 on Axonal Guidance"*
**作者**: Lee S, et al.
**摘要**: 通过体外表达不同磷酸化状态的重组CRMP1.证实其磷酸化修饰可改变Semaphorin信号传导，进而影响轴突导向的化学排斥反应。
---
如需获取真实文献，建议在PubMed或Google Scholar中检索关键词“CRMP1 recombinant protein”或结合具体研究主题(如“CRMP1 structure/phosphorylation/neuronal function”)。
**Background of CRMP1 Recombinant Protein**
Collapsin response mediator protein 1 (CRMP1) is a member of the CRMP family (CRMP1-5), which plays critical roles in neuronal development, including axon guidance, neurite outgrowth, and synaptic plasticity. Initially identified as a downstream effector of the collapsin/semaphorin signaling pathway, CRMP1 regulates cytoskeletal dynamics by interacting with tubulin heterodimers and modulating microtubule assembly. This protein is highly expressed in the nervous system, particularly during early brain development, and its activity is tightly regulated by phosphorylation through kinases such as glycogen synthase kinase-3β (GSK-3β) and cyclin-dependent kinase 5 (Cdk5).
CRMP1 recombinant protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian cell systems, to produce a purified, functional form for experimental studies. Its recombinant version enables researchers to investigate CRMP1’s molecular mechanisms in vitro and in vivo, such as its role in neuronal polarity, dendritic spine formation, and interactions with signaling partners like semaphorin 3A. Dysregulation of CRMP1 has been implicated in neurological disorders, including Alzheimer’s disease, where aberrant phosphorylation contributes to tau pathology and synaptic dysfunction.
Additionally, CRMP1 is studied in cancer due to its potential role in metastasis, as it influences cell migration and invasion. The recombinant protein serves as a tool for drug discovery, aiding in screening compounds that modulate CRMP1 activity or its pathological interactions. Structural studies using recombinant CRMP1 have also clarified its domain organization and post-translational modification sites, providing insights into its functional diversity. Overall, CRMP1 recombinant protein is vital for advancing understanding of neurodevelopment, neurodegeneration, and cancer biology, offering therapeutic potential for related diseases.
×
