| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIAS3 |
| Uniprot No | Q9Y6X2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-628aa |
| 氨基酸序列 | MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL LAPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST SSQRFEEAHF TFALTPQQVQ QILTSREVLP GAKCDYTIQV QLRFCLCETS CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILSS CSDCDEIQFM EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SPVQGGDPSE NKKKVEVIDL TIESSSDEED LPPTKKHCSV TSAAIPALPG SKGVLTSGHQ PSSVLRSPAM GTLGGDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP GRVSSIVAPG GALREGHGGP LPSGPSLTGC RSDIISLD |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与PIAS3重组蛋白相关的参考文献概览：
1. **"PIAS3 suppresses NF-κB-mediated transcription by interacting with the p65/RelA subunit"**
*作者：Liu et al.*
摘要：研究利用重组PIAS3蛋白验证其通过直接结合NF-κB亚基p65.抑制炎症基因转录的分子机制，揭示其在炎症调控中的作用。
2. **"Recombinant PIAS3 protein attenuates STAT3 signaling and exhibits antitumor activity in vitro"**
*作者：Shin et al.*
摘要：通过纯化重组PIAS3蛋白，证实其抑制STAT3磷酸化及下游肿瘤基因表达，在癌细胞系中诱导凋亡，提示其作为STAT3靶向治疗分子的潜力。
3. **"Purification and functional characterization of PIAS3 as a SUMO-E3 ligase for p53"**
*作者：Megidish et al.*
摘要：报道重组PIAS3蛋白的纯化方法，并证明其介导p53的SUMO化修饰，调节p53在DNA损伤反应中的稳定性与转录活性。
4. **"PIAS3 modulates HIF-1α transcriptional activity in hypoxia through SUMOylation"**
*作者：Carbia-Nagashima et al.*
摘要：利用重组PIAS3蛋白发现其在低氧条件下通过SUMO化修饰HIF-1α，调控血管生成相关基因表达，为肿瘤微环境研究提供新视角。
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**注**：以上文献为模拟概括，实际引用需核对具体论文内容。建议通过PubMed或Web of Science以“PIAS3 AND (recombinant OR purification)”为关键词检索最新文献。
**Background of PIAS3 Recombinant Protein**
PIAS3 (Protein Inhibitor of Activated STAT3) is a member of the PIAS family, a group of proteins that regulate transcriptional responses by modulating the activity of STAT (Signal Transducer and Activator of Transcription) signaling pathways. Discovered as a specific suppressor of STAT3-mediated gene expression, PIAS3 interacts directly with activated STAT3 dimers, blocking their DNA-binding ability and suppressing transcriptional activation. Beyond STAT3. PIAS3 also regulates other transcription factors, including NF-κB and p53. through SUMOylation (post-translational modification via small ubiquitin-like modifiers) or direct protein-protein interactions.
Structurally, PIAS3 contains conserved domains critical for its function: an N-terminal SAP domain (involved in chromatin binding), a central zinc-binding motif, and a C-terminal SUMO E3 ligase domain. Its role in cellular processes spans immune regulation, inflammation, apoptosis, and oncogenesis. Dysregulation of PIAS3 is linked to diseases such as cancer, autoimmune disorders, and neurodegenerative conditions, where it may act as either a tumor suppressor or promoter, depending on context.
Recombinant PIAS3 proteins are engineered for research to study its molecular mechanisms, interactions, and therapeutic potential. Produced via bacterial or mammalian expression systems, these proteins retain functional domains and are used in assays like pull-down experiments, SUMOylation studies, and inhibitor screening. Recent studies explore PIAS3 as a target for cancer therapy, as modulating its activity could restore STAT3 regulation or enhance anti-tumor immunity. However, challenges remain in understanding its dual roles and cell-specific effects.
Overall, PIAS3 recombinant tools are vital for dissecting its complex biology and advancing translational applications in precision medicine.
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