| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP21 |
| Uniprot No | Q8N119 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 145-569aa |
| 氨基酸序列 | SPRAPL SLSRRGWQPR GYPDGGAAQA FSKRTLSWRL LGEALSSQLS VADQRRIVAL AFRMWSEVTP LDFREDLAAP GAAVDIKLGF GRGRHLGCPR AFDGSGQEFA HAWRLGDIHF DDDEHFTPPT SDTGISLLKV AVHEIGHVLG LPHTYRTGSI MQPNYIPQEP AFELDWSDRK AIQKLYGSCE GSFDTAFDWI RKERNQYGEV MVRFSTYFFR NSWYWLYENR NNRTRYGDPI QILTGWPGIP THNIDAFVHI WTWKRDERYF FQGNQYWRYD SDKDQALTED EQGKSYPKLI SEGFPGIPSP LDTAFYDRRQ KLIYFFKESL VFAFDVNRNR VLNSYPKRIT EVFPAVIPQN HPFRNIDSAY YSYAYNSIFF FKGNAYWKVV NDKDKQQNSW LPANGLFPKK FISEKWFDVC DVHISTLNM |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP21重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库查询确认)：
1. **标题**：*Expression and functional characterization of recombinant human MMP21 in embryonic development*
**作者**：Smith A, et al.
**摘要**：本研究在大肠杆菌系统中成功表达并纯化重组人MMP21蛋白，通过体外酶活实验证实其具有金属蛋白酶活性，并发现其在斑马鱼胚胎发育中参与神经嵴细胞迁移调控。
2. **标题**：*MMP21 promotes tumor invasion via recombinant protein-mediated ECM remodeling*
**作者**：Li X, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度MMP21重组蛋白，体外实验表明其能降解细胞外基质成分(如胶原IV)，并增强乳腺癌细胞的侵袭能力，提示其在肿瘤转移中的潜在作用。
3. **标题**：*Structural insights into MMP21 catalytic domain by X-ray crystallography*
**作者**：Zhang Y, et al.
**摘要**：通过重组MMP21催化结构域的结晶与结构解析，揭示了其独特的锌离子结合位点及底物特异性机制，为设计靶向抑制剂提供结构基础。
4. **标题**：*MMP21 recombinant protein rescues craniofacial defects in a mouse model*
**作者**：Garcia-Ruiz C, et al.
**摘要**：在MMP21基因敲除小鼠模型中，外源性重组MMP21蛋白注射显著改善颅面骨发育异常，证实其在组织形态发生中的关键功能。
**提示**：实际文献检索建议使用关键词“MMP21 recombinant protein”在PubMed或Web of Science中查询，并优先选择近5年发表的论文以获取最新进展。
**Background of MMP21 Recomcombinant Protein**
Matrix metalloproteinase 21 (MMP21) is a member of the zinc-dependent endopeptidase family, which plays critical roles in extracellular matrix (ECM) remodeling, tissue development, and disease progression. Unlike many MMPs, MMP21 is classified as a "matrylisin" subfamily member due to its unique structural features, including a conserved catalytic domain and a propeptide region. It is expressed during embryogenesis and implicated in cell migration, organogenesis, and immune regulation, though its precise biological functions remain less characterized compared to other MMPs.
Recombinant MMP21 protein is produced using biotechnological systems (e.g., *E. coli*, mammalian cells, or insect cells*) to enable controlled study of its enzymatic activity and interactions. The recombinant form typically retains the catalytic domain and may include tags (e.g., His-tag) for purification. Researchers utilize it to investigate MMP21's substrate specificity, regulatory mechanisms (e.g., interaction with TIMPs), and involvement in pathological conditions such as cancer metastasis, fibrosis, and congenital disorders. For example, MMP21 overexpression has been linked to tumor invasion and abnormal tissue remodeling, making it a potential therapeutic target.
Current challenges in MMP21 research include elucidating its physiological substrates and signaling pathways, as well as optimizing recombinant protein stability and activity for functional assays. Advances in structural biology and gene-editing tools are aiding in clarifying its role in development and disease. As a relatively understudied MMP, recombinant MMP21 serves as a vital tool for bridging knowledge gaps and exploring its therapeutic or diagnostic potential in precision medicine.
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