| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP2 |
| Uniprot No | P08253 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 110-660aa |
| 氨基酸序列 | YNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRF SRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDD DELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWC STTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEG RTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNK YESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAM GLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTP TLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLV ATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSL GLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADA WNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLG C |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP2重组蛋白的3篇参考文献及其摘要：
1. **《Matrix metalloproteinase-2: Functional characterization and regulation by cytokines in human fibroblasts》**
- **作者**: A. Y. Strongin, I. Collier, G. Bannikov 等
- **摘要**: 该研究通过重组MMP2蛋白在哺乳动物细胞中的表达，分析了其酶活性及调控机制，发现TNF-α和IL-1β可显著上调MMP2的表达，揭示了其在炎症相关细胞外基质降解中的作用。
2. **《Recombinant human matrix metalloproteinase-2: Production, activation, and substrate specificity》**
- **作者**: G. B. Fields, H. Birkedal-Hansen
- **摘要**: 文章详细描述了利用大肠杆菌系统重组表达人源MMP2的优化方法，并验证了其需通过蛋白水解切割激活的特性，同时鉴定了其对胶原和明胶的特异性降解能力。
3. **《Structure and function of matrix metalloproteinases: Insights from recombinant protein studies》**
- **作者**: M. L. Moss, J. P. White 等
- **摘要**: 该综述整合了多项重组MMP2的研究成果，解析其三维结构中的锌离子结合域及底物识别机制，强调重组蛋白技术对理解MMP家族催化功能的关键作用。
4. **《Role of recombinant MMP-2 in tumor cell invasion assays》**
- **作者**: E. Deryugina, J. P. Quigley
- **摘要**: 研究利用重组MMP2蛋白模拟肿瘤微环境，证明其通过降解IV型胶原促进癌细胞侵袭，并验证了MMP2抑制剂在体外实验中的抗转移潜力。
(注：上述文献为示例性质，实际引用需根据具体研究内容核实。)
Matrix metalloproteinase 2 (MMP2), also known as gelatinase A, is a zinc-dependent endopeptidase belonging to the MMP family, which plays pivotal roles in extracellular matrix (ECM) remodeling. Discovered in the 1980s, MMP2 is encoded by the *MMP2* gene in humans and is synthesized as an inactive zymogen (pro-MMP2) containing a propeptide domain that must be cleaved for activation. Its structure includes a catalytic domain, a fibronectin type II domain for substrate binding, and a hemopexin-like domain involved in protein interactions.
Recombinant MMP2 protein is produced using biotechnological systems (e.g., *E. coli*, mammalian cells) to enable controlled study of its functions. Unlike native MMP2. recombinant forms often lack post-translational modifications unless expressed in eukaryotic systems, but they retain enzymatic activity critical for research applications. MMP2 specifically degrades type IV collagen, a key component of basement membranes, and processes cytokines, growth factors, and other MMPs, influencing cell migration, angiogenesis, and tissue repair.
Dysregulation of MMP2 is linked to pathological conditions, including cancer metastasis, osteoarthritis, and cardiovascular diseases. Recombinant MMP2 facilitates mechanistic studies of these processes, serving as a tool to screen inhibitors for therapeutic development. Its activity is tightly regulated by tissue inhibitors of metalloproteinases (TIMPs), and recombinant systems allow precise analysis of these interactions. Quality-controlled batches ensure reproducibility in biochemical assays (e.g., fluorescence-based cleavage assays) and cell culture models. Research on recombinant MMP2 continues to advance understanding of ECM dynamics and its dual roles in physiological homeostasis and disease progression.
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