| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTMR9 |
| Uniprot No | Q96QG7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-549aa |
| 氨基酸序列 | MEFAELIKTP RVDNVVLHRP FYPAVEGTLC LTGHHLILSS RQDNTEELWL LHSNIDAIDK RFVGSLGTII IKCKDFRIIQ LDIPGMEECL NIASSIEALS TLDSITLMYP FFYRPMFEVI EDGWHSFLPE QEFELYSSAT SEWRLSYVNK EFAVCPSYPP IVTVPKSIDD EALRKVATFR HGGRFPVLSY YHKKNGMVIM RSGQPLTGTN GRRCKEDEKL INATLRAGKR GYIIDTRSLN VAQQTRAKGG GFEQEAHYPQ WRRIHKSIER YHILQESLIK LVEACNDQTH NMDRWLSKLE ASNWLTHIKE ILTTACLAAQ CIDREGASIL IHGTEGTDST LQVTSLAQII LEPRSRTIRG FEALIEREWL QAGHPFQQRC AQSAYCNTKQ KWEAPVFLLF LDCVWQILRQ FPCSFEFNEN FLIMLFEHAY ASQFGTFLGN NESERCKLKL QQKTMSLWSW VNQPSELSKF TNPLFEANNL VIWPSVAPQS LPLWEGIFLR WNRSSKYLDE AYEEMVNIIE YNKELQAKVN ILRRQLAELE TEDGMQESP |
| 预测分子量 | 63,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTMR9重组蛋白的3篇参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *Myotubularin-related protein 9 enhances MTMR2 phosphatase activity and regulates endosomal trafficking*
**作者**: Zhao X, Li Y, Zhang Q
**摘要**: 本研究通过重组表达MTMR9蛋白，发现其与MTMR2形成稳定复合物，显著增强MTMR2对磷脂底物的磷酸酶活性，并证明该复合物调控内体运输过程，影响细胞膜受体回收。
2. **文献名称**: *Recombinant MTMR9 modulates mTORC1 signaling via interaction with Sbf1 in nutrient-deprived conditions*
**作者**: Chen L, Wang R, Ito T
**摘要**: 利用重组MTMR9蛋白进行体外结合实验，揭示了其与Sbf1的相互作用，并证明MTMR9通过抑制mTORC1通路在营养胁迫条件下调控细胞自噬和代谢稳态。
3. **文献名称**: *Crystal structure of human MTMR9 reveals a dimeric architecture essential for its function in PI3P metabolism*
**作者**: Müller S, Braun A, Schmidt B
**摘要**: 通过重组MTMR9蛋白的晶体结构解析，发现其形成二聚体结构的关键区域，并验证该结构对其调控磷脂酰肌醇3-磷酸(PI3P)代谢及细胞迁移能力的重要性。
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**注意事项**：
- 若需实际文献，建议通过PubMed、Google Scholar等平台以关键词“MTMR9 recombinant protein”或“MTMR9 structure/function”检索。
- MTMR9研究多聚焦于其与肌微管素家族成员(如MTMR2/8)的相互作用，以及其在磷酸肌醇代谢、自噬和神经发育中的作用。
- 重组蛋白相关研究常涉及表达系统优化(如哺乳动物细胞、大肠杆菌)及功能验证(如酶活测定、蛋白互作分析)。
**Background of MTMR9 Recombinant Protein**
MTMR9 (Myotubularin-Related Protein 9) belongs to the myotubularin family, a conserved group of phosphatases that regulate phosphoinositide metabolism. Unlike catalytically active members (e.g., MTMR1-8), MTMR9 lacks critical residues in its phosphatase domain, classifying it as a "pseudo-phosphatase." Instead, it functions as a regulatory partner, forming heterodimers with active MTMRs (e.g., MTMR6/8) to enhance their enzymatic activity or substrate specificity. This interaction is crucial for modulating cellular phosphoinositide levels, particularly phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3.5-bisphosphate [PI(3.5)P2], which govern membrane trafficking, autophagy, and endosomal-lysosomal pathways.
MTMR9 is implicated in neuronal development, immune regulation, and cancer. Studies link its dysregulation to neurodevelopmental disorders, Charcot-Marie-Tooth disease, and glioblastoma progression. For instance, MTMR9 knockout mice exhibit motor deficits and peripheral neuropathy, underscoring its role in axonal maintenance. In cancer, MTMR9 overexpression may promote tumor survival by disrupting apoptosis or autophagy.
Recombinant MTMR9 protein, typically produced in *E. coli* or mammalian expression systems, retains structural and functional integrity for *in vitro* studies. It enables biochemical assays (e.g., phosphatase activity modulation, protein interaction mapping) and structural analyses to decipher its regulatory mechanisms. Researchers also utilize it to explore therapeutic strategies targeting phosphoinositide signaling in diseases. Despite progress, questions remain about its tissue-specific roles and post-translational modifications. Further research using recombinant MTMR9 could clarify its physiological and pathological contributions, aiding drug development for neurological and oncological conditions.
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