| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CARNS1 |
| Uniprot No | A5YM72 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-827aa |
| 氨基酸序列 | MLLCLSPAWLMKVPAPGQPGEAALLVSKAVSFHPGGLTFLDDFVPPRRATYFLAGLGLGPGRGREAAELARDLTCPTGASAELARLLEDRLLTRQLLAQQGGVAVPATLAFTYKPPGLLRGGDASLGLRLVELSGKEGQETLVKEEVEAFLRSEALGDILQVAVKLSGWRWRGRQAWRLHPRAELGAVVDTVLALLEKLEEEESVLVEAVYPPAQLPCSDGPSPGPGLAVRICAVVCRTQGDRPLLSKVVCGVGRGDRPLRHHNSLPRTLEVALAQCGLGEEAQVAAVRQRVKAAAEAALAAVLALEAGLSAEQRGGRRAHTDFLGVDFALTAAGGVLTPVALELNGGLCLEACGALEGLWAAPRLGPAADEAVAAPLVETMLRRSARCLMEGKQLLVVGAGGVSKKFVWEAARDYGLQLHLVESDPNHFASQLVQTFIHFDMTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAAMRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK |
| 预测分子量 | 88,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CARNS1重组蛋白的模拟参考文献示例(注：部分文献信息为假设性概括，实际研究需通过学术数据库验证)：
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1. **标题**：*Expression and Purification of Recombinant Human CARNS1 in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：本研究报道了人源CARNS1蛋白在大肠杆菌中的重组表达与纯化。通过优化密码子使用和诱导条件，成功获得可溶性CARNS1蛋白，并利用镍柱亲和层析纯化。酶活性分析表明，重组蛋白具有催化肌肽合成的功能。
2. **标题**：*Functional Characterization of CARNS1 in Cellular Redox Homeostasis*
**作者**：Zhang L, et al.
**摘要**：通过哺乳动物细胞表达重组CARNS1.探究其在氧化应激中的作用。实验发现，过表达CARNS1可显著降低活性氧(ROS)水平，提示其在抗氧化防御中的潜在应用。
3. **标题**：*Structural Insights into CARNS1 Catalytic Mechanism via X-ray Crystallography*
**作者**：Johnson R, et al.
**摘要**：本研究解析了重组CARNS1蛋白的晶体结构(分辨率2.1 Å)，揭示了其底物结合口袋的关键氨基酸残基，为设计靶向抑制剂提供了结构基础。
4. **标题**：*CARNS1 Deficiency Model Generated by CRISPR/Cas9 in Zebrafish*
**作者**：Wang Y, et al.
**摘要**：利用CRISPR/Cas9技术构建斑马鱼CARNS1基因敲除模型，并补充重组CARNS1蛋白后，发现其肌肉发育缺陷表型部分恢复，证实了CARNS1在肌肽代谢中的生理功能。
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**建议**：实际文献检索可通过PubMed、Web of Science等平台，以关键词“CARNS1 recombinant protein”或结合具体研究领域(如酶学、代谢疾病)进行深入查询。
CARNS1 (Carnosine Synthase 1), also known as ATPGD1. is an enzyme encoded by the *CARNS1* gene in humans. It plays a critical role in synthesizing carnosine (β-alanyl-L-histidine), a naturally occurring dipeptide with antioxidant, pH-buffering, and anti-glycation properties. Carnosine is highly abundant in skeletal muscle and brain tissues, where it contributes to cellular homeostasis and protects against oxidative stress and metabolic acidosis. CARNS1 catalyzes the ATP-dependent conjugation of β-alanine and L-histidine to form carnosine, a reaction essential for maintaining tissue-specific carnosine levels.
Recombinant CARNS1 protein is produced using biotechnological platforms, such as bacterial (e.g., *E. coli*) or mammalian expression systems, to enable large-scale purification and functional studies. The recombinant form retains enzymatic activity, allowing researchers to investigate its kinetic properties, substrate specificity, and regulatory mechanisms. Its production has facilitated structural studies, including crystallography, to elucidate active-site architecture and catalytic mechanisms.
Research on CARNS1 has gained momentum due to its potential therapeutic relevance. Carnosine depletion is linked to aging, diabetic complications, and neurodegenerative disorders like Alzheimer’s disease. Recombinant CARNS1 serves as a tool to explore enzyme replacement strategies or enhance carnosine biosynthesis in pathological conditions. Additionally, it aids in developing inhibitors or modulators targeting carnosine metabolism for metabolic or neuromuscular diseases. Current studies also focus on CARNS1 polymorphisms and their association with carnosine variability in human populations, highlighting its role as a biomarker for health and disease. Overall, recombinant CARNS1 bridges biochemical research and translational applications in redox biology and precision medicine.
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