| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NPR3 |
| Uniprot No | P17342 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-481aa |
| 氨基酸序列 | GGVGGGGGGAGIGGGRQEREALPPQKIEVLVLLPQDDSYLFSLTRVRPAIEYALRSVEGNGTGRRLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAAAPVARLASHWDLPMLSAGALAAGFQHKDSEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALVYSDDKLERNCYFTLEGVHEVFQEEGLHTSIYSFDETKDLDLEDIVRNIQASERVVIMCASSDTIRSIMLVAHRHGMTSGDYAFFNIELFNSSSYGDGSWKRGDKHDFEAKQAYSSLQTVTLLRTVKPEFEKFSMEVKSSVEKQGLNMEDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVIAMTDVEAGTQEVIGDYFGKEGRFEMRPNVKYPWGPLKLRIDENRIVEHTNSSPCKSSGGLEE |
| 预测分子量 | 53.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NPR3重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression and Characterization of Recombinant Human Natriuretic Peptide Receptor 3 (NPR3) in Mammalian Cells"*
**作者**：Smith A, et al.
**摘要**：该研究通过哺乳动物细胞系统成功表达并纯化人源NPR3重组蛋白，证实其与心房钠尿肽(ANP)的高亲和力结合，并揭示其在细胞外液调节中的潜在作用。
2. **文献名称**：*"Structural Insights into NPR3-C Ligand Specificity by X-ray Crystallography"*
**作者**：Lee C, et al.
**摘要**：通过X射线晶体学解析NPR3重组蛋白胞外结构域的三维结构，阐明其选择性结合C型钠尿肽(CNP)的分子机制，为靶向药物设计提供依据。
3. **文献名称**：*"NPR3 Modulates Vascular Smooth Muscle Cell Proliferation via cGMP-Independent Pathways"*
**作者**：Wang Y, et al.
**摘要**：研究发现重组NPR3蛋白通过非cGMP依赖的信号通路抑制血管平滑肌细胞增殖，提示其在动脉粥样硬化治疗中的潜在应用价值。
注：以上文献信息为示例，实际文献需通过学术数据库(如PubMed)检索确认。
**Background of NPR3 Recombinant Protein**
NPR3 (Natriuretic Peptide Receptor 3), also known as the natriuretic peptide clearance receptor, is a member of the natriuretic peptide receptor family. It plays a critical role in regulating cardiovascular and renal homeostasis by binding and clearing natriuretic peptides (NPs), including atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). Unlike NPR1 and NPR2. which mediate signaling through guanylyl cyclase activity, NPR3 lacks intrinsic enzymatic function and primarily modulates NP levels via internalization and degradation, thereby fine-tuning their physiological effects.
Recombinant NPR3 protein is engineered using biotechnological methods, often expressed in mammalian or insect cell systems to ensure proper post-translational modifications, such as glycosylation, which are essential for ligand binding and receptor stability. This engineered protein retains the extracellular ligand-binding domain, enabling its use in structural studies, ligand-receptor interaction assays, and drug discovery.
Research on NPR3 has gained momentum due to its dual role in cardiovascular diseases and metabolic disorders. Elevated NPR3 levels are linked to hypertension, heart failure, and obesity, making it a potential therapeutic target or biomarker. Recombinant NPR3 facilitates the study of NP clearance mechanisms, aids in screening inhibitors to modulate NP bioavailability, and supports the development of diagnostic tools. Its application extends to understanding pathological pathways and evaluating novel treatments aimed at enhancing natriuretic peptide signaling, a promising strategy for managing cardiometabolic conditions.
In summary, NPR3 recombinant protein serves as a vital tool for unraveling the complexities of NP regulation and advancing translational research in cardiovascular and metabolic health.
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