| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STS |
| Uniprot No | P08842 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-583aa |
| 氨基酸序列 | MPLRKMKIPFLLLFFLWEAESHAASRPNIILVMADDLGIGDPGCYGNKTIRTPNIDRLASGGVKLTQHLAASPLCTPSRAAFMTGRYPVRSGMASWSRTGVFLFTASSGGLPTDEITFAKLLKDQGYSTALIGKWHLGMSCHSKTDFCHHPLHHGFNYFYGISLTNLRDCKPGEGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLHVPLGVFFSLLFLAALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQRNTETPFLLVLSYLHVHTALFSSKDFAGKSQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYFTSDQGAHVEEVSSKGEIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEPTSNMDIFPTVAKLAGAPLPEDRIIDGRDLMPLLEGKSQRSDHEFLFHYCNAYLNAVRWHPQNSTSIWKAFFFTPNFNPVGSNGCFATHVCFCFGSYVTHHDPPLLFDISKDPRERNPLTPASEPRFYEILKVMQEAADRHTQTLPEVPDQFSWNNFLWKPWLQLCCPSTGLSCQCDREKQDKRLSR |
| 预测分子量 | 65,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于STS重组蛋白的3-4条示例参考文献(内容为虚构，仅供格式参考)：
1. **文献名称**：*Expression and Purification of Recombinant Human STS Protein in E. coli*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌表达系统高效表达重组人STS蛋白，通过亲和层析技术纯化，验证其酶活性，为后续药物开发提供基础。
2. **文献名称**：*Structural Analysis of STS Recombinant Protein for Drug Design*
**作者**：Lee B, et al.
**摘要**：通过X射线晶体学解析STS重组蛋白的三维结构，揭示其底物结合位点，为基于结构的抑制剂设计提供理论依据。
3. **文献名称**：*Functional Characterization of Yeast-Expressed STS in Steroid Metabolism*
**作者**：Garcia C, et al.
**摘要**：在毕赤酵母系统中表达STS重组蛋白，分析其在类固醇代谢中的功能，证实其催化硫酸酯水解活性与疾病治疗潜力。
4. **文献名称**：*Optimization of STS Recombinant Production for Industrial Applications*
**作者**：Wang D, et al.
**摘要**：比较不同表达系统(哺乳动物细胞、昆虫细胞)生产STS重组蛋白的效率和成本，提出工业化大规模生产的优化策略。
**注意**：以上文献为示例，实际引用时需检索权威数据库(如PubMed、Web of Science)获取真实文献信息。
**Background of STS Recombinant Proteins**
STS (Staphylococcal Superantigen-like Toxins) recombinant proteins are engineered versions of virulence factors produced by *Staphylococcus aureus*, a pathogenic bacterium responsible for diverse infections. These toxins belong to the superantigen-like protein (SSL) family, which shares structural homology with superantigens but exhibits distinct immune evasion mechanisms. Unlike classical superantigens that hyperactivate T-cells, STS proteins primarily disrupt host immune responses by targeting innate immune components, such as complement proteins, neutrophils, or immunoglobulins, to facilitate bacterial survival and colonization.
The development of recombinant STS proteins leverages genetic engineering to express and purify these toxins in heterologous systems (e.g., *E. coli* or yeast). This approach enables precise study of their molecular interactions, structure-function relationships, and pathogenic roles. For instance, STS proteins like SSL10 or SSL7 have been shown to inhibit complement activation by binding to IgG or C5. respectively, providing insights into immune evasion strategies.
Recombinant STS proteins are pivotal in biomedical research, serving as tools to dissect host-pathogen interactions, screen inhibitory compounds, or design vaccines. Their controlled production also supports structural studies (e.g., X-ray crystallography) to identify therapeutic targets. However, challenges remain in mimicking post-translational modifications and ensuring functional fidelity during recombinant expression.
Overall, STS recombinant proteins bridge basic science and translational applications, offering a pathway to novel anti-staphylococcal therapies and deepening our understanding of bacterial pathogenesis.
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