| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DAPP1 |
| Uniprot No | Q9UN19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-280aa |
| 氨基酸序列 | MGRAELLEGKMSTQDPSDLWSRSDGEAELLQDLGWYHGNLTRHAAEALLL SNGCDGSYLLRDSNETTGLYSLSVRAKDSVKHFHVEYTGYSFKFGFNEFS SLKDFVKHFANQPLIGSETGTLMVLKHPYPRKVEEPSIYESVRVHTAMQT GRTEDDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLHRNELKYFKDQM SPEPIRILDLTECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEW IKILRWKLSQIRKQLNQGEGTIRSRSFIFK |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DAPP1重组蛋白的3篇参考文献示例(内容基于公开研究概括，非真实文献，仅供参考)：
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1. **文献名称**：*DAPP1 modulates B-cell receptor signaling via phosphorylation of adaptor molecules*
**作者**：Smith A, et al.
**摘要**：研究利用重组DAPP1蛋白，揭示了其在B细胞受体信号通路中的调控作用，发现DAPP1通过磷酸化下游接头蛋白(如BLNK)影响B细胞活化与增殖。
2. **文献名称**：*Structural insights into the DAPP1 PH domain interaction with phosphatidylinositol lipids*
**作者**：Chen L, et al.
**摘要**：通过重组表达DAPP1蛋白的PH结构域，结合X射线晶体学分析，阐明了其与磷脂酰肌醇3.4.5-三磷酸(PIP3)的特异性结合机制，为免疫细胞趋化性研究提供结构基础。
3. **文献名称**：*DAPP1 deficiency impairs T-cell immune responses in a murine model*
**作者**：Wang Y, et al.
**摘要**：利用重组DAPP1蛋白回补实验，验证了DAPP1缺失导致T细胞活化障碍，表明其在TCR信号传导中通过调控AKT/mTOR通路影响免疫应答。
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注：以上文献为示例，实际研究中请通过PubMed或Web of Science检索真实文献。
**Background of DAPP1 Recombinant Protein**
DAPP1 (Dual Adaptor for Phosphotyrosine and 3-Phosphoinositides), also known as Bam32. is a signaling protein involved in regulating immune cell responses, particularly in B lymphocytes. It contains two critical functional domains: an N-terminal SH2 (Src homology 2) domain that binds phosphorylated tyrosine residues and a C-terminal PH (pleckstrin homology) domain that interacts with phosphatidylinositol (3.4.5)-trisphosphate (PIP3), a lipid secondary messenger generated by phosphoinositide 3-kinase (PI3K). These domains enable DAPP1 to act as a scaffold, coordinating downstream signaling events triggered by B-cell receptor (BCR) activation.
DAPP1 plays a role in modulating immune cell proliferation, survival, and migration. Studies highlight its involvement in PI3K-dependent pathways, where it influences the activation of kinases like Akt and regulates calcium signaling. Dysregulation of DAPP1 has been linked to immune disorders and cancers, underscoring its importance in maintaining cellular homeostasis.
Recombinant DAPP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce a purified, bioactive form for research. It serves as a tool to study molecular interactions, signaling mechanisms, and drug targeting in vitro. By analyzing DAPP1’s structure-function relationships, researchers aim to uncover its role in diseases and explore therapeutic interventions. Its recombinant form is vital for assays investigating immune receptor signaling, protein-protein interactions, and inhibitor screening, contributing to advancements in immunology and oncology research.
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