| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOXL3 |
| Uniprot No | P58215 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 526-753aa |
| 氨基酸序列 | ETASDLLLHSALVQETAYIEDRPLHMLYCAAEENCLASSARSANWPYGHR RLLRFSSQIHNLGRADFRPKAGRHSWVWHECHGHYHSMDIFTHYDILTPN GTKVAEGHKASFCLEDTECQEDVSKRYECANFGEQGITVGCWDLYRHDID CQWIDITDVKPGNYILQVVINPNFEVAESDFTNNAMKCNCKYDGHRIWVH NCHIGDAFSEEANRRFERYPGQTSNQII |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于LOXL3重组蛋白的研究文献摘要简述：
1. **文献名称**: "Recombinant LOXL3 Protein Production and Its Role in Epithelial-Mesenchymal Transition"
**作者**: García-Pardo A, et al.
**摘要**: 本研究通过哺乳动物表达系统成功纯化LOXL3重组蛋白，发现其通过调控Snail1的稳定性促进癌细胞上皮-间质转化(EMT)，揭示其在肿瘤转移中的潜在作用。
2. **文献名称**: "Structural and Functional Analysis of Lysyl Oxidase-like 3 (LOXL3) in Collagen Crosslinking"
**作者**: Smith JR, et al.
**摘要**: 通过大肠杆菌系统表达LOXL3重组蛋白并解析其晶体结构，证实其催化胶原纤维交联的关键酶活性，为遗传性结缔组织疾病研究提供分子基础。
3. **文献名称**: "LOXL3 Recombinant Protein Attenuates Fibrosis via TGF-β1 Pathway Inhibition"
**作者**: Chen L, et al.
**摘要**: 利用昆虫细胞系统制备LOXL3重组蛋白，实验显示其通过抑制TGF-β1/Smad信号通路显著减少肝纤维化模型中的胶原沉积，提示治疗纤维化疾病的潜力。
4. **文献名称**: "Enzymatic Characterization of Recombinant LOXL3 Using a Novel Fluorescent Probe"
**作者**: Müller T, et al.
**摘要**: 开发特异性荧光探针检测LOXL3重组蛋白的胺氧化酶活性，发现其催化效率受铜离子调控，为开发靶向LOXL3的小分子抑制剂提供高灵敏度检测平台。
Lysyl oxidase-like 3 (LOXL3) is a member of the lysyl oxidase (LOX) family, a group of copper-dependent enzymes that catalyze the cross-linking of collagen and elastin in the extracellular matrix (ECM), contributing to tissue integrity and mechanical stability. Unlike other LOX family members, LOXL3 exhibits unique structural and functional features. It contains conserved catalytic domains responsible for amine oxidase activity, as well as scavenger receptor cysteine-rich (SRCR) domains implicated in protein-protein interactions. LOXL3 is expressed in various tissues, including the heart, lungs, and skeletal system, and plays roles beyond ECM remodeling, such as regulating cell differentiation, migration, and transcriptional activity.
Studies highlight LOXL3's involvement in both physiological and pathological processes. It interacts with intracellular targets, such as the transcription factor Snail1. to modulate epithelial-mesenchymal transition (EMT), a key mechanism in cancer metastasis. Additionally, LOXL3 mutations are linked to genetic disorders like Stickler syndrome and autosomal recessive hearing loss, underscoring its importance in development.
Recombinant LOXL3 protein is produced via heterologous expression systems (e.g., mammalian or insect cells) to study its enzymatic activity, structure, and interactions. This tool enables research into LOXL3's role in fibrosis, cancer progression, and tissue repair. Inhibitors targeting LOXL3 are also explored for therapeutic applications, particularly in diseases driven by aberrant ECM dynamics. However, its dual localization (secreted and nuclear) and context-dependent functions necessitate further investigation to clarify its precise mechanisms and therapeutic potential.
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