| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KDM4C |
| Uniprot No | Q9H3R0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-460aa |
| 氨基酸序列 | MEVAEVESPL NPSCKIMTFR PSMEEFREFN KYLAYMESKG AHRAGLAKVI PPKEWKPRQC YDDIDNLLIP APIQQMVTGQ SGLFTQYNIQ KKAMTVKEFR QLANSGKYCT PRYLDYEDLE RKYWKNLTFV APIYGADING SIYDEGVDEW NIARLNTVLD VVEEECGISI EGVNTPYLYF GMWKTTFAWH TEDMDLYSIN YLHFGEPKSW YAIPPEHGKR LERLAQGFFP SSSQGCDAFL RHKMTLISPS VLKKYGIPFD KITQEAGEFM ITFPYGYHAG FNHGFNCAES TNFATVRWID YGKVAKLCTC RKDMVKISMD IFVRKFQPDR YQLWKQGKDI YTIDHTKPTP ASTPEVKAWL QRRRKVRKAS RSFQCARSTS KRPKADEEEE VSDEVDGAEV PNPDSVTDDL KVSEKSEAAV KLRNTEASSE EESSASRMQV EQNLSDHIKL SGNSCLSTSV |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural basis of the recognition of a methylated histone tail by JMJD2C"** by Ng, S.S. et al.
- 该研究解析了KDM4C(JMJD2C)与甲基化组蛋白复合物的晶体结构，阐明了其底物识别机制，并利用重组蛋白验证了其去甲基化活性。
2. **"KDM4C regulates TGF-β signaling and promotes osteosarcoma progression"** by Zhang, Y. et al.
- 通过重组KDM4C蛋白进行体外酶活实验，揭示了其通过调控TGF-β通路促进骨肉瘤细胞侵袭的分子机制。
3. **"A high-throughput screen identifies inhibitors of the interaction between PHF8 and H3K9me3"** by Horton, J.R. et al.
- 研究中利用重组KDM4C蛋白进行酶活抑制筛选，发现小分子抑制剂可特异性阻断其H3K9me3去甲基化功能，为癌症治疗提供潜在靶点。
4. **"JMJD2C promotes colorectal cancer metastasis via regulating histone methylation of NOTCH promoters"** by Wang, L. et al.
- 通过表达纯化的重组KDM4C蛋白，证实其通过去甲基化H3K9me3激活NOTCH信号通路，驱动结直肠癌转移。
注：以上文献名为示例，实际引用时需核实真实发表信息。
**Background of KDM4C Recombinant Protein**
KDM4C (lysine-specific demethylase 4C), also known as JMJD2C or GASC1. is a member of the Jumonji C (JmjC) domain-containing histone demethylase family. It catalyzes the removal of methyl groups from histone H3 lysine 9 (H3K9me3/me2) and H3 lysine 36 (H3K36me3/me2), modulating chromatin structure and gene expression. This enzyme plays a critical role in epigenetic regulation, influencing cellular processes such as proliferation, differentiation, DNA repair, and stem cell maintenance.
KDM4C is implicated in various cancers, including breast, prostate, and leukemia, where its overexpression correlates with tumor progression, metastasis, and therapy resistance. By demethylating repressive histone marks, KDM4C promotes oncogene activation, suppresses tumor suppressors, and enhances genomic instability. Its dysregulation is also linked to developmental disorders and metabolic diseases.
Recombinant KDM4C protein is engineered for *in vitro* studies to dissect its enzymatic mechanisms, substrate specificity, and interactions with inhibitors. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form retains catalytic activity dependent on Fe²⁺ and α-ketoglutarate cofactors. Researchers utilize it for high-throughput drug screening, structural analysis (e.g., X-ray crystallography), and functional assays to explore its role in disease pathways.
As a therapeutic target, KDM4C inhibition shows promise in preclinical models, driving interest in developing small-molecule inhibitors or epigenetic-based therapies. Its recombinant form thus serves as a vital tool for advancing both basic research and translational applications in oncology and epigenetics.
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