| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TK1 |
| Uniprot No | P04183 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSCINL PTVLPGSPSK TRGQIQVILG PMFSGKSTEL MRRVRRFQIA QYKCLVIKYA KDTRYSSSFC THDRNTMEAL PACLLRDVAQ EALGVAVIGI DEGQFFPDIV EFCEAMANAG KTVIVAALDG TFQRKPFGAI LNLVPLAESV VKLTAVCMEC FREAAYTKRL GTEKEVEVIG GADKYHSVCR LCYFKKASGQ PAGPDNKENC PVPGKPGEAV AARKLFAPQQ ILQCSPAN |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TK1重组蛋白的3篇代表性文献摘要简述：
1. **文献名称**: "Production and characterization of recombinant human thymidine kinase 1"
**作者**: Jagarlamudi S, Eriksson S
**摘要**: 研究报道了通过大肠杆菌表达系统重组表达人源TK1蛋白，优化纯化方法并验证其酶学活性，为癌症诊断试剂开发提供基础。
2. **文献名称**: "Development of a sensitive ELISA using recombinant TK1 for early cancer detection"
**作者**: Zhang J, et al.
**摘要**: 利用重组TK1蛋白构建新型免疫检测方法，证实其在乳腺癌患者血清中的检测灵敏度显著提升，验证其作为肿瘤生物标志物的潜力。
3. **文献名称**: "Structural insights into recombinant TK1 from Drosophila melanogaster"
**作者**: Müller M, et al.
**摘要**: 通过昆虫细胞表达系统获得果蝇TK1重组蛋白，解析其晶体结构并揭示底物结合位点特征，为抗肿瘤药物设计提供结构依据。
注：以上为基于领域知识的示例性内容，实际文献需通过PubMed/Google Scholar检索关键词"recombinant thymidine kinase 1"获取。近年研究多聚焦于TK1重组蛋白在液体活检和靶向治疗中的应用拓展。
**Background of Recomcombinant TK1 Protein**
Thymidine kinase 1 (TK1) is a cytosolic enzyme involved in the salvage pathway of DNA synthesis, catalyzing the phosphorylation of thymidine to thymidine monophosphate. Primarily expressed during the S-phase of the cell cycle, TK1 plays a critical role in cell proliferation and is closely associated with DNA replication and repair. Unlike its mitochondrial counterpart, TK2. TK1 is cell cycle-regulated and exhibits elevated activity in rapidly dividing cells, including cancer cells. This property has made TK1 a promising biomarker for cancer diagnosis, prognosis, and therapeutic monitoring, particularly in hematologic malignancies and solid tumors.
Recombinant TK1 protein is engineered through genetic cloning techniques, where the *TK1* gene is inserted into expression vectors (e.g., *E. coli* or mammalian cell systems) to produce purified, functional protein. This approach ensures high yield, consistency, and reduced batch-to-batch variability compared to native TK1 isolated from biological tissues. Recombinant TK1 retains enzymatic activity and antigenic properties, making it invaluable for both research and clinical applications.
In research, recombinant TK1 is used to study cell cycle dynamics, DNA metabolism, and mechanisms of carcinogenesis. Clinically, it serves as a key component in immunoassays (e.g., ELISA) to quantify TK1 levels in serum, aiding early cancer detection and monitoring treatment responses. Additionally, it supports drug development by enabling high-throughput screening of TK1-targeted anticancer agents. Recent studies also explore its role in viral infections, as some viruses hijack host TK1 for replication.
Overall, recombinant TK1 bridges basic science and clinical innovation, offering tools to advance oncology and molecular biology. Its utility underscores the importance of enzyme dynamics in health and disease.
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