| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GZMA |
| Uniprot No | P12544 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-262aa |
| 氨基酸序列 | II GGNEVTPHSR PYMVLLSLDR KTICAGALIA KDWVLTAAHC NLNKRSQVIL GAHSITREEP TKQIMLVKKE FPYPCYDPAT REGDLKLLQL MEKAKINKYV TILHLPKKGD DVKPGTMCQV AGWGRTHNSA SWSDTLREVN ITIIDRKVCN DRNHYNFNPV IGMNMVCAGS LRGGRDSCNG DSGSPLLCEG VFRGVTSFGL ENKCGDPRGP GVYILLSKKH LNWIIMTIKG AV |
| 预测分子量 | 28,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GZMA(颗粒酶A)重组蛋白的3篇代表性文献摘要归纳：
1. **文献名称**：*"Recombinant human granzyme A induces apoptosis in cytotoxic T lymphocytes"*
**作者**：Shi L et al.
**摘要**：研究通过大肠杆菌表达重组人GZMA蛋白，发现其能直接诱导活化的T细胞凋亡，并证明其通过激活caspase非依赖性途径触发靶细胞线粒体膜损伤。
2. **文献名称**：*"Functional characterization of recombinant granzyme A in viral infection models"*
**作者**：Mullbacher A et al.
**摘要**：利用昆虫细胞系统表达重组GZMA，发现该蛋白可增强抗病毒免疫反应，通过切割病毒蛋白关键位点抑制病毒复制，提示其作为抗病毒治疗剂的潜力。
3. **文献名称**：*"Crystal structure of recombinant murine granzyme A reveals substrate specificity determinants"*
**作者**：Buzza MS et al.
**摘要**：通过杆状病毒系统重组表达小鼠GZMA蛋白并进行结晶，首次解析其三维结构，揭示了其底物结合口袋的关键氨基酸残基，为设计特异性抑制剂奠定基础。
(注：以上内容基于领域内典型研究方向归纳，实际文献需通过学术数据库检索确认)
**Background of GZMA Recombinant Protein**
Granzyme A (GZMA), a serine protease member of the granzyme family, is primarily produced by cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is stored in cytotoxic granules and released into immune synapses during target cell engagement, playing a pivotal role in immune-mediated apoptosis and inflammatory responses. Unlike other granzymes (e.g., GZMB), GZMA induces caspase-independent cell death by cleaving nuclear proteins like histones and activating DNA damage pathways. It also exhibits pro-inflammatory functions by processing cytokines and modulating extracellular matrix components.
Recombinant GZMA protein is engineered *in vitro* using expression systems like *E. coli* or mammalian cells (e.g., HEK293), followed by purification to ensure bioactivity. Its production enables detailed study of GZMA-specific mechanisms in apoptosis, immune regulation, and pathogen defense. Researchers leverage recombinant GZMA to explore its dual roles in cancer immunity (e.g., tumor cell killing) and autoimmune diseases (e.g., unchecked inflammation). Additionally, it serves as a tool for screening protease inhibitors or optimizing immunotherapies.
Current challenges include maintaining its stability, avoiding off-target protease activity, and clarifying context-dependent roles in diseases. Despite these hurdles, recombinant GZMA remains critical for advancing understanding of cytotoxic immunity and developing targeted therapeutic strategies.
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