| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRHBP |
| Uniprot No | P24387 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-322aa |
| 氨基酸序列 | YLELREAADYDPFLLFSANLKRELAGEQPYRRALRCLDMLSLQGQFTFTA DRPQLHCAAFFISEPEEFITIHYDQVSIDCQGGDFLKVFDGWILKGEKFP SSQDHPLPSAERYIDFCESGLSRRSIRSSQNVAMIFFRVHEPGNGFTLTI KTDPNLFPCNVISQTPNGKFTLVVPHQHRNCSFSIIYPVVIKISDLTLGH VNGLQLKKSSAGCEGIGDFVELLGGTGLDPSKMTPLADLCYPFHGPAQMK VGCDNTVVRMVSSGKHVNRVTFEYRQLEPYELENPNGNSIGEFCLSGLVD HHHHHH |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRHBP(促肾上腺皮质激素释放激素结合蛋白)重组蛋白的示例参考文献(注：以下内容为模拟虚构，仅供参考)：
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1. **文献名称**: *Expression and Purification of Recombinant CRHBP in E. coli: Functional Characterization*
**作者**: Smith A, et al. (2016)
**摘要**: 本研究报道了在大肠杆菌系统中高效表达重组CRHBP蛋白的优化方法，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，重组CRHBP能有效结合CRH并抑制其下游信号通路活性，为后续结构功能研究提供基础。
2. **文献名称**: *Structural Insights into CRHBP-CRH Interaction by X-ray Crystallography*
**作者**: Brown C, et al. (2018)
**摘要**: 通过X射线晶体学解析了重组CRHBP与CRH的复合物结构，揭示了关键结合位点及构象变化，阐明了CRHBP调控CRH生物利用度的分子机制，为靶向该通路的药物设计提供依据。
3. **文献名称**: *Recombinant CRHBP Attenuates Stress-Induced Behaviors in Rodent Models*
**作者**: Lee J, et al. (2020)
**摘要**: 在小鼠模型中，外源性重组CRHBP显著降低慢性应激诱导的焦虑样行为，并调节HPA轴活性，提示其作为治疗应激相关疾病的潜在治疗剂。
4. **文献名称**: *CRHBP Modulates Placental CRH Signaling in Human Pregnancy*
**作者**: Zhang Y, et al. (2021)
**摘要**: 利用重组CRHBP蛋白，研究证实其在胎盘组织中通过结合游离CRH调控早产风险，体外实验表明重组蛋白可抑制CRH介导的炎症因子释放。
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**注**：以上文献为示例，实际引用需根据具体研究检索PubMed、Google Scholar等数据库获取真实文献。
**Background of CRHBP Recombinant Protein**
Corticotropin-releasing hormone-binding protein (CRHBP) is a secreted glycoprotein that modulates the bioavailability and activity of corticotropin-releasing hormone (CRH), a key neuropeptide in the hypothalamic-pituitary-adrenal (HPA) axis. CRH regulates stress responses, appetite, and immune function by binding to its receptors (CRHR1/2). CRHBP acts as a natural buffer, binding CRH with high affinity to limit receptor activation, thereby fine-tuning stress and inflammatory signaling.
Structurally, CRHBP belongs to the CRH family, featuring a conserved N-terminal domain and a C-terminal β-sheet-rich region critical for ligand interaction. It binds CRH and related peptides like urocortins, influencing their stability and signaling. Dysregulation of CRHBP is linked to stress-related disorders (e.g., depression, anxiety), metabolic syndromes, and conditions like Cushing’s disease.
Recombinant CRHBP is produced using expression systems such as *E. coli* or mammalian cells (e.g., HEK293), ensuring proper folding and post-translational modifications. Purification techniques like affinity chromatography yield high-purity protein for research. This recombinant tool is vital for studying CRH-CRHR interactions, screening therapeutic agents, and modeling diseases. For example, it helps explore CRHBP mutations affecting stress responses or investigate CRH overexpression in pathologies.
In drug development, CRHBP serves as a target or delivery vehicle for modulating CRH pathways. Its recombinant form also aids in structural studies, such as crystallography, to map binding interfaces and design CRHR antagonists. Overall, CRHBP recombinant protein is indispensable for unraveling HPA axis dynamics and advancing therapies for neuroendocrine and stress-related disorders.
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