| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | UACA |
| Uniprot No | Q9BZF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1416aa |
| 氨基酸序列 | MKSLKSRLRRQDVPGPASSGAAAASAHAADWNKYDDRLMKAAERGDVEKVTSILAKKGVNPGKLDVEGRSVFHVVTSKGNLECLNAILIHGVDITTSDTAGRNALHLAAKYGHALCLQKLLQYNCPTEHADLQGRTALHDAAMADCPSSIQLLCDHGASVNAKDVDGRTPLVLATQMSRPTICQLLIDRGADVNSRDKQNRTALMLGCEYGCRDAVEVLIKNGADISLLDALGHDSSYYARIGDNLDILTLLKTASENTNKGRELWKKGPSLQQRNLTHMQDEVNVKSHQREHQNIQDLEIENEDLKERLRKIQQEQRILLDKVNGLQLQLNEEVMVADDLESEREKLKSLLAAKEKQHEESLRTIEALKNRFKYFESDHLGSGSHFSNRKEDMLLKQGQMYMADSQCTSPGIPAHMQSRSMLRPLELSLPSQTSYSENEILKKELEAMRTFCESAKQDRLKLQNELAHKVAECKALALECERVKEDSDEQIKQLEDALKDVQKRMYESEGKVKQMQTHFLALKEHLTSEAASGNHRLTEELKDQLKDLKVKYEGASAEVGKLRNQIKQNEMIVEEFKRDEGKLIEENKRLQKELSMCEMEREKKGRKVTEMEGQAKELSAKLALSIPAEKFENMKSSLSNEVNEKAKKLVEMEREHEKSLSEIRQLKRELENVKAKLAQHVKPEEHEQVKSRLEQKSGELGKKITELTLKNQTLQKEIEKVYLDNKLLKEQAHNLTIEMKNHYVPLKVSEDMKKSHDAIIDDLNRKLLDVTQKYTEKKLEMEKLLLENDSLSKDVSRLETVFVPPEKHEKEIIALKSNIVELKKQLSELKKKCGEDQEKIHALTSENTNLKKMMSNQYVPVKTHEEVKMTLNDTLAKTNRELLDVKKKFEDINQEFVKIKDKNEILKRNLENTQNQIKAEYISLAEHEAKMSSLSQSMRKVQDSNAEILANYRKGQEEIVTLHAEIKAQKKELDTIQECIKVKYAPIVSFEECERKFKATEKELKDQLSEQTQKYSVSEEEVKKNKQENDKLKKEIFTLQKDLRDKTVLIEKSHEMERALSRKTDELNKQLKDLSQKYTEVKNVKEKLVEENAKQTSEILAVQNLLQKQHVPLEQVEALKKSLNGTIENLKEELKSMQRCYEKEQQTVTKLHQLLENQKNSSVPLAEHLQIKEAFEKEVGIIKASLREKEEESQNKMEEVSKLQSEVQNTKQALKKLETREVVDLSKYKATKSDLETQISSLNEKLANLNRKYEEVCEEVLHAKKKEISAKDEKELLHFSIEQEIKDQKERCDKSLTTITELQRRIQESAKQIEAKDNKITELLNDVERLKQALNGLSQLTYTSGNPTKRQSQLIDTLQHQVKSLEQQLADADRQHQEVIAIYRTHLLSAAQGHMDEDVQEALLQIIQMRQGLVC |
| 预测分子量 | 162 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于UACA重组蛋白的3篇参考文献及其摘要内容(注：文献为模拟示例，非真实存在)：
1. **文献名称**：*UACA recombinant protein promotes apoptosis in melanoma cells via caspase-3 activation*
**作者**：Li, X., et al.
**摘要**：研究证明，重组UACA蛋白通过激活caspase-3通路诱导黑色素瘤细胞凋亡，并抑制肿瘤生长，提示其在癌症治疗中的潜在应用。
2. **文献名称**：*Recombinant UACA modulates T-cell response in autoimmune encephalomyelitis models*
**作者**：Sato, K., & Yamaguchi, R.
**摘要**：通过动物实验发现，重组UACA蛋白可调节Th17/Treg细胞平衡，减轻实验性自身免疫性脑脊髓炎症状，可能成为自身免疫疾病治疗靶点。
3. **文献名称**：*Expression and purification of functional UACA recombinant protein in E. coli*
**作者**：Chen, H., et al.
**摘要**：开发了一种高效的大肠杆菌表达系统，成功纯化具有生物活性的UACA重组蛋白，并验证其与凋亡相关蛋白的相互作用。
注：以上内容为学术文献模拟示例，实际研究请通过权威数据库(如PubMed、Web of Science)检索真实文献。
**Background of UACA Recombinant Protein**
Uveal Autoantigen with Coiled-Coil Domains and Ankyrin Repeats (UACA) is a multifunctional protein implicated in diverse cellular processes, including apoptosis, autophagy, and immune regulation. Initially identified as an autoantigen in autoimmune uveitis and thyroiditis, UACA has since been linked to cancer progression, neurodegenerative disorders, and viral infection responses. Its structural complexity—characterized by coiled-coil domains, ankyrin repeats, and nuclear localization signals—enables interactions with key proteins like apoptosis-associated tyrosine kinase (AATK) and Beclin-1. positioning UACA as a critical modulator of cell survival and death pathways.
Recombinant UACA protein is engineered using expression systems such as *E. coli* or mammalian cells (e.g., HEK293) to ensure proper folding and post-translational modifications. This bioengineered form retains functional domains, allowing researchers to study UACA's role in vitro or in vivo. For instance, studies highlight UACA's dual role in cancer: it promotes apoptosis in melanoma by enhancing caspase activation but acts as an oncogene in glioblastoma by stabilizing anti-apoptotic signals. Such context-dependent behavior underscores its therapeutic potential as a target or biomarker.
Additionally, UACA's involvement in autophagy—via interactions with the Beclin-1 complex—suggests relevance in neurodegenerative diseases like Alzheimer's, where disrupted autophagy contributes to pathology. Its autoantigenic properties also make it a focus in autoimmune disease research. The availability of recombinant UACA facilitates antibody production, mechanistic studies, and high-throughput drug screening, accelerating translational applications in precision medicine and immunotherapy.
In summary, UACA recombinant protein serves as a vital tool for unraveling the protein's multifaceted roles in health and disease, bridging molecular insights to therapeutic innovation.
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