| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGC |
| Uniprot No | P35626 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-688aa |
| 氨基酸序列 | MADLEAVLAD VSYLMAMEKS KATPAARASK RIVLPEPSIR SVMQKYLAER NEITFDKIFN QKIGFLLFKD FCLNEINEAV PQVKFYEEIK EYEKLDNEED RLCRSRQIYD AYIMKELLSC SHPFSKQAVE HVQSHLSKKQ VTSTLFQPYI EEICESLRGD IFQKFMESDK FTRFCQWKNV ELNIHLTMNE FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRIKMK QGETLALNER IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDLH YHLSQHGVFS EKEMRFYATE IILGLEHMHN RFVVYRDLKP ANILLDEHGH ARISDLGLAC DFSKKKPHAS VGTHGYMAPE VLQKGTAYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTVN VELPDTFSPE LKSLLEGLLQ RDVSKRLGCH GGGSQEVKEH SFFKGVDWQH VYLQKYPPPL IPPRGEVNAA DAFDIGSFDE EDTKGIKLLD CDQELYKNFP LVISERWQQE VTETVYEAVN ADTDKIEARK RAKNKQLGHE EDYALGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQNL LTMEQILSVE ETQIKDKKCI LFRIKGGKQF VLQCESDPEF VQWKKELNET FKEAQRLLRR APKFLNKPRS GTVELPKPSL CHRNSNGL |
| 预测分子量 | 79,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AGC重组蛋白研究的参考文献示例(内容为虚构，仅供格式参考)：
1. **《Recombinant expression and characterization of human AGC kinase family members in E. coli》**
- 作者：Smith J. et al.
- 摘要：研究通过大肠杆菌系统优化表达人源AGC激酶(如AKT、PKA等)，分析其酶活性和磷酸化调控机制，为药物筛选提供纯化蛋白工具。
2. **《Structural insights into AGC kinase activation through recombinant protein crystallography》**
- 作者：Chen L. & Wang Y.
- 摘要：利用重组蛋白技术解析AGC激酶PDK1的晶体结构，揭示其C端疏水模体在变构激活中的关键作用，指导靶向抑制剂设计。
3. **《High-yield production of functional AGC kinases using mammalian cell expression systems》**
- 作者：Rodriguez M. et al.
- 摘要：比较哺乳动物细胞与昆虫细胞表达系统对AGC激酶(如PKCγ)活性的影响，证实哺乳动物系统更利于保留天然构象和底物识别功能。
4. **《AGC kinase recombinant variants for targeted cancer therapy screening》**
- 作者：Kim S. & Tanaka H.
- 摘要：构建AGC家族激酶(如SGK1)的致癌突变体重组蛋白，用于高通量筛选特异性小分子抑制剂，验证其在肿瘤细胞凋亡中的调控效应。
注：以上文献信息为模拟生成，实际引用请检索PubMed或SciFinder数据库获取真实研究。
**Background of AGC Recombinant Proteins**
The AGC family of protein kinases, named after its founding members PKA (Protein Kinase A), PKG (Protein Kinase G), and PKC (Protein Kinase C), represents a critical group of enzymes involved in cellular signaling. These kinases share conserved structural motifs, including a catalytic domain regulated by secondary domains or interacting partners, and play pivotal roles in processes such as metabolism, proliferation, and apoptosis. Dysregulation of AGC kinases is implicated in diseases like cancer, diabetes, and neurological disorders, making them key therapeutic targets.
Recombinant AGC proteins are engineered using genetic techniques to express and purify these kinases in heterologous systems (e.g., *E. coli*, insect, or mammalian cells*). This approach allows large-scale production of functional, post-translationally modified kinases for research and drug development. Recombinant technology enables precise modifications, such as activating mutations or phosphorylation mimics, to study structure-function relationships or simulate disease states.
In drug discovery, recombinant AGC kinases are used for high-throughput screening of inhibitors or activators. Structural studies (e.g., X-ray crystallography, cryo-EM) leveraging recombinant proteins have revealed mechanisms of kinase activation and regulation, guiding the design of selective therapeutics. Challenges remain in preserving native conformation and activity during recombinant production, particularly for kinases requiring complex regulatory interactions.
Recent advances in protein engineering, such as baculovirus systems or cell-free expression, aim to improve yield and functionality. Additionally, recombinant AGC proteins are vital in studying crosstalk between signaling pathways (e.g., PI3K/AKT/mTOR) and developing targeted therapies. Their versatility continues to drive innovations in both basic research and clinical applications.
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