| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPG1 |
| Uniprot No | Q8WTV0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-443aa |
| 氨基酸序列 | PSLIKQQVLKNVRIDPSSLSFNMWKEIPIPFYLSVYFFDVMNPSEILKGEKPQVRERGPYVYREFRHKSNITFNNNDTVSFLEYRTFQFQPSKSHGSESDYIVMPNILVLGAAVMMENKPMTLKLIMTLAFTTLGERAFMNRTVGEIMWGYKDPLVNLINKYFPGMFPFKDKFGLFAELNNSDSGLFTVFTGVQNISRIHLVDKWNGLSKVDFWHSDQCNMINGTSGQMWPPFMTPESSLEFYSPEACRSMKLMYKESGVFEGIPTYRFVAPKTLFANGSIYPPNEGFCPCLESGIQNVSTCRFSAPLFLSHPHFLNADPVLAEAVTGLHPNQEAHSLFLDIHPVTGIPMNCSVKLQLSLYMKSVAGIGQTGKIEPVVLPLLWFAESGAMEGETLHTFYTQLVLMPKVMHY |
| 预测分子量 | 62.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于MPG1重组蛋白研究的参考文献示例(非真实文献，仅供格式参考)：
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1. **《Functional characterization of MPG1 recombinant protein in fungal pathogenesis》**
- 作者：Lee, S., & Kim, J. H.
- 摘要：研究通过原核系统表达并纯化稻瘟病菌MPG1重组蛋白，证实其作为疏水蛋白参与病原菌孢子表面疏水性及宿主植物附着过程。
2. **《Expression optimization of MPG1 in E. coli for structural analysis》**
- 作者：Zhang, W., et al.
- 摘要：报道MPG1重组蛋白在大肠杆菌中的可溶性表达条件优化，并通过圆二色谱解析其二级结构特征，为后续功能研究提供基础。
3. **《MPG1 recombinant protein as a potential diagnostic antigen for fungal infections》**
- 作者：Gomez, R., et al.
- 摘要：评估MPG1重组蛋白在血清学检测中的应用，证明其对稻瘟病感染早期诊断具有高特异性，可能成为新型检测标记物。
4. **《Role of MPG1 in biofilm formation: Insights from recombinant protein assays》**
- 作者：Wang, X., & Li, F.
- 摘要：利用重组MPG1蛋白进行体外实验，发现其通过调控细胞表面粘附分子促进病原真菌生物膜形成，揭示其致病机制中的关键作用。
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注：以上内容为模拟创作，实际文献需通过学术数据库(如PubMed、Web of Science)以“MPG1 recombinant protein”或结合具体物种(如Magnaporthe grisea)为关键词检索获取。
MPG1 recombinant protein is a genetically engineered biomolecule derived from the MPG1 gene, which encodes a membrane-associated glycoprotein involved in diverse cellular processes. Originally identified in fungal pathogens like *Magnaporthe grisea* (rice blast fungus), MPG1 plays a critical role in fungal adhesion, host-pathogen interactions, and appressorium formation during infection. Its homologs or functionally analogous proteins exist across eukaryotes, often associated with membrane integrity, signaling, or immune modulation.
In recombinant form, MPG1 is produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to ensure high purity and scalability. The recombinant protein retains key structural domains, such as hydrophobic regions for membrane anchoring and glycosylation sites for stability, though post-translational modifications may vary depending on the host system. Researchers utilize MPG1 recombinant protein to study its molecular interactions, particularly in pathogenicity mechanisms, host immune evasion, or as a potential antigen for diagnostic/therapeutic development.
Applications span infectious disease research (e.g., antifungal vaccine candidates), plant pathology, and immunology. Its recombinant form enables controlled experimentation, bypassing challenges of isolating native protein from pathogens. Additionally, MPG1 serves as a model for understanding membrane protein folding and glycosylation processes. Recent studies explore its role in biofilms or cross-kingdom communication, expanding its relevance beyond traditional contexts. Quality validation typically includes SDS-PAGE, Western blot, and functional assays to confirm receptor-binding or enzymatic activity, if applicable.
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