| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AVPR2 |
| Uniprot No | P30518 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MLMASTTSAVPGHPSLPSLPSNSSQERPLDTRDPLLARAELALLSIVFVAVALSNGLVLAALARRGRRGHWAPIHVFIGHLCLADLAVALFQVLPQLAWKATDRFRGPDALCRAVKYLQMVGMYASSYMILAMTLDRHRAICRPMLAYRHGSGAHWNRPVLVAWAFSLLLSLPQLFIFAQRNVEGGSGVTDCWACFAEPWGRRTYVTWIALMVFVAPTLGIAACQVLIFREIHASLVPGPSERPGGRRRGRRTGSPGEGAHVSAAVAKTVRMTLVIVVVYVLCWAPFFLVQLWAAWDPEAPLEGAPFVLLMLLASLNSCTNPWIYASFSSSVSSELRSLLCCARGRTPPSLGPQDESCTTASSSLAKDTSS |
| 预测分子量 | 40,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于AVPR2重组蛋白的文献信息，按研究重点分类整理：
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### 1. **《Functional characterization of the human V2 vasopressin receptor mutations in nephrogenic diabetes insipidus》**
**作者**: Rosenthal W. et al. (1993)
**摘要**: 通过在大肠杆菌中重组表达AVPR2突变体，研究其与肾源性尿崩症的关系，发现部分突变导致受体无法正常偶联G蛋白，影响cAMP信号通路。
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### 2. **《Expression and Purification of the Human Vasopressin V2 Receptor for Structural Studies》**
**作者**: Krumm B.E. et al. (2019)
**摘要**: 利用昆虫细胞-杆状病毒系统重组表达AVPR2蛋白，优化纯化流程并通过冷冻电镜解析其三维结构，为靶向药物设计提供依据。
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### 3. **《Development of a Bioluminescence Resonance Energy Transfer Assay to Study Ligand-Specific Signaling via the AVPR2》**
**作者**: Charest-Morin X. et al. (2015)
**摘要**: 构建AVPR2重组蛋白的BRET(生物发光共振能量转移)检测系统，分析不同配体激活受体后下游信号通路的差异性，揭示潜在治疗策略。
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**领域关联性**：
以上研究覆盖了AVPR2重组蛋白的疾病机制(文献1)、结构解析(文献2)及药物开发工具(文献3)，反映了该领域从基础到应用的典型研究方向。如需更多文献或特定主题(如表达系统优化)，可进一步补充。
**Background of AVPR2 Recombinant Protein**
The arginine vasopressin receptor 2 (AVPR2), a member of the G protein-coupled receptor (GPCR) family, plays a critical role in regulating water homeostasis in the kidneys. It is primarily expressed in the renal collecting duct cells, where it binds to antidiuretic hormone (ADH, or vasopressin) to activate intracellular signaling pathways. Upon ligand binding, AVPR2 stimulates the Gs protein-adenylyl cyclase cascade, increasing cyclic AMP (cAMP) levels. This triggers the translocation of aquaporin-2 (AQP2) water channels to the apical membrane, facilitating water reabsorption and urine concentration.
Mutations in the *AVPR2* gene are linked to X-linked nephrogenic diabetes insipidus (NDI), a disorder characterized by the kidney's inability to concentrate urine, leading to excessive thirst and dehydration. Studying AVPR2's structure and function is vital for understanding NDI pathophysiology and developing targeted therapies.
Recombinant AVPR2 protein is engineered using heterologous expression systems, such as mammalian or insect cell lines, to produce functional receptors for *in vitro* studies. This protein retains ligand-binding specificity and signaling capabilities, enabling research on receptor-ligand interactions, signaling mechanisms, and drug screening. It is widely used to investigate NDI-associated mutations, test potential agonists/antagonists, and explore GPCR modulation strategies. The development of AVPR2 recombinant protein has significantly advanced both basic research and therapeutic innovation in renal and endocrine disorders.
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