| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AVPR1B |
| Uniprot No | P47901 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-424aa |
| 氨基酸序列 | MDSGPLWDANPTPRGTLSAPNATTPWLGRDEELAKVEIGVLATVLVLATGGNLAVLLTLGQLGRKRSRMHLFVLHLALTDLAVALFQVLPQLLWDITYRFQGPDLLCRAVKYLQVLSMFASTYMLLAMTLDRYLAVCHPLRSLQQPGQSTYLLIAAPWLLAAIFSLPQVFIFSLREVIQGSGVLDCWADFGFPWGPRAYLTWTTLAIFVLPVTMLTACYSLICHEICKNLKVKTQAWRVGGGGWRTWDRPSPSTLAATTRGLPSRVSSINTISRAKIRTVKMTFVIVLAYIACWAPFFSVQMWSVWDKNAPDEDSTNVAFTISMLLGNLNSCCNPWIYMGFNSHLLPRPLRHLACCGGPQPRMRRRLSDGSLSSRHTTLLTRSSCPATLSLSLSLTLSGRPRPEESPRDLELADGEGTAETIIF |
| 预测分子量 | 53.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AVPR1B重组蛋白的3篇参考文献及其简要摘要：
1. **《Expression and functional characterization of the human vasopressin V1b receptor in mammalian cells》**
- **作者**: Saito, M., et al.
- **摘要**: 本研究通过在大鼠肾细胞中表达人类AVPR1B重组蛋白，分析了其与配体结合特性及信号传导机制。发现该受体通过Gq蛋白激活磷脂酶C通路，并揭示了其与应激相关激素的相互作用。
2. **《Characterization of a novel vasopressin V1b receptor antagonist, SSR149415. in rodent models of anxiety》**
- **作者**: Serradeil-Le Gal, C., et al.
- **摘要**: 研究利用重组AVPR1B蛋白筛选出一种选择性拮抗剂SSR149415.证明其通过阻断受体活性显著减少焦虑行为，为AVPR1B在情绪障碍中的作用提供了药理学证据。
3. **《Structural basis for ligand recognition and activation of the vasopressin V1b receptor》**
- **作者**: Wang, Y., et al.
- **摘要**: 通过冷冻电镜技术解析了AVPR1B重组蛋白与精氨酸加压素结合的复合物结构，揭示了配体结合口袋的关键氨基酸残基，为靶向该受体的药物设计提供了结构基础。
(注：以上文献信息为示例，实际引用需根据具体研究核实准确性。)
**Background of AVPR1B Recombinant Protein**
The arginine vasopressin receptor 1B (AVPR1B), also known as V1bR, is a G protein-coupled receptor (GPCR) that binds arginine vasopressin (AVP) and plays a critical role in regulating stress responses, social behaviors, and endocrine functions. Predominantly expressed in the pituitary gland, brain, and peripheral tissues, AVPR1B activates intracellular signaling pathways, primarily via Gq-mediated phospholipase C activation, leading to calcium mobilization and protein kinase C activation.
AVPR1B is implicated in modulating the hypothalamic-pituitary-adrenal (HPA) axis by promoting adrenocorticotropic hormone (ACTH) release from the pituitary, thereby influencing cortisol secretion and stress adaptation. Dysregulation of AVPR1B signaling is associated with neuropsychiatric disorders (e.g., depression, anxiety) and endocrine pathologies like Cushing’s syndrome.
Recombinant AVPR1B proteins are engineered in vitro using expression systems (e.g., mammalian, insect cells) to study receptor-ligand interactions, screen therapeutic compounds, and elucidate molecular mechanisms. These proteins often include tags (e.g., His, FLAG) for purification and detection. Research on AVPR1B recombinant variants aids in developing targeted therapies, such as selective antagonists (e.g., SSR149415) for stress-related disorders, highlighting its translational potential in precision medicine.
Overall, AVPR1B recombinant proteins serve as vital tools for advancing both basic research and drug discovery in neuroendocrinology and behavioral sciences.
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