| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CREBZF |
| Uniprot No | Q9NS37 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMRHSLTK LLAASGSNSP TRSESPEPAA TCSLPSDLTR AAAGEEETAA AGSPGRKQQF GDEGELEAGR GSRGGVAVRA PSPEEMEEEA IASLPGEETE DMDFLSGLEL ADLLDPRQPD WHLDPGLSSP GPLSSSGGGS DSGGLWRGDD DDEAAAAEMQ RFSDLLQRLL NGIGGCSSSS DSGSAEKRRR KSPGGGGGGG SGNDNNQAAT KSPRKAAAAA ARLNRLKKKE YVMGLESRVR GLAAENQELR AENRELGKRV QALQEESRYL RAVLANETGL ARLLSRLSGV GLRLTTSLFR DSPAGDHDYA LPVGKQKQDL LEEDDSAGGV CLHVDKDKVS VEFCSACARK ASSSLKM |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CREBZF重组蛋白的3篇参考文献示例(注：文献信息为模拟内容，仅供参考)：
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1. **Structural insights into CREBZF as a transcriptional regulator**
*Zhang, Y. et al. (2015). Journal of Biological Chemistry.*
摘要：本研究通过重组表达并纯化人源CREBZF蛋白，利用X射线晶体学解析其DNA结合域的三维结构，揭示了其与特定启动子序列结合的分子机制，为理解其在转录调控中的作用提供了结构基础。
2. **CREBZF interacts with SREBP1 to regulate hepatic lipid metabolism**
*Li, H. et al. (2018). Biochemical and Biophysical Research Communications.*
摘要：通过在大肠杆菌中重组表达CREBZF蛋白，结合免疫共沉淀实验，发现其与SREBP1直接相互作用，并在肝脏细胞中调控脂质代谢相关基因的表达，提示其在代谢疾病中的潜在功能。
3. **Recombinant CREBZF exhibits stress-dependent nuclear translocation**
*Wang, Q. et al. (2020). Cellular Signalling.*
摘要：研究利用重组CREBZF蛋白和荧光标记技术，证明其在氧化应激条件下通过特定磷酸化位点调控核质穿梭，进而影响下游基因表达，揭示了其在细胞应激响应中的动态调控机制。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“CREBZF recombinant protein”或结合具体研究方向筛选。
**Background of CREBZF Recombinant Protein**
CREBZF (CREB/ATF bZIP transcription factor), also known as SMILE or ZHX2. is a member of the basic leucine zipper (bZIP) transcription factor family. It contains a unique N-terminal zinc-finger domain (ZID) and a C-terminal region homologous to the CREB-binding domain, enabling interactions with other transcription regulators. CREBZF functions as a transcriptional modulator, influencing gene expression by competing with or cooperating with CREB, ATF, and AP-1 family proteins. It plays roles in diverse cellular processes, including metabolism, proliferation, differentiation, and stress responses.
The recombinant CREBZF protein is engineered for research applications, typically produced in *E. coli* or mammalian expression systems to ensure proper folding and post-translational modifications. Its production involves cloning the CREBZF gene into expression vectors, followed by purification via affinity chromatography. Recombinant CREBZF is widely used to study its molecular mechanisms, such as its involvement in the cAMP/PKA and PI3K/AKT signaling pathways, and its regulatory effects on genes like *PEPCK* and *G6Pase* in glucose metabolism.
Recent studies highlight CREBZF's dual role in cancer, acting as a tumor suppressor or promoter depending on cellular context. It also interacts with nuclear receptors (e.g., LXR, PPARγ) to modulate lipid homeostasis. Researchers employ recombinant CREBZF in *in vitro* assays (e.g., EMSA, luciferase reporter), protein-protein interaction studies, and cell-based models to explore its therapeutic potential in metabolic disorders, neurodegeneration, and oncology. Its versatility makes it a critical tool for unraveling CREBZF's physiological and pathological significance.
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