| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GP2 |
| Uniprot No | P55259 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-537aa |
| 氨基酸序列 | MPHLMERMVGSGLLWLALVSCILTQASAVQRGYGNPIEASSYGLDLDCGAPGTPEAHVCFDPCQNYTLLDEPFRSTENSAGSQGCDKNMSGWYRFVGEGGVRMSETCVQVHRCQTDAPMWLNGTHPALGDGITNHTACAHWSGNCCFWKTEVLVKACPGGYHVYRLEGTPWCNLRYCTVPRDPSTVEDKCEKACRPEEECLALNSTWGCFCRQDLNSSDVHSLQPQLDCGPREIKVKVDKCLLGGLGLGEEVIAYLRDPNCSSILQTEERNWVSVTSPVQASACRNILERNQTHAIYKNTLSLVNDFIIRDTILNINFQCAYPLDMKVSLQAALQPIVSSLNVSVDGNGEFIVRMALFQDQNYTNPYEGDAVELSVESVLYVGAILEQGDTSRFNLVLRNCYATPTEDKADLVKYFIIRNSCSNQRDSTIHVEENGQSSESRFSVQMFMFAGHYDLVFLHCEIHLCDSLNEQCQPSCSRSQVRSEVPAIDLARVLDLGPITRRGAQSPGVMNGTPSTAGFLVAWPMVLLTVLLAWLF |
| 预测分子量 | 59,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于GP2重组蛋白的参考文献及其摘要概括：
1. **"Autoantibodies against the exocrine pancreas in autoimmune pancreatitis: A study of a combined German and Italian cohort"**
- **作者**: Löhr, J.M. et al.
- **摘要**: 研究分析了GP2重组蛋白作为自身抗原在自身免疫性胰腺炎(AIP)和其他自身免疫疾病中的作用，发现GP2抗体与原发性硬化性胆管炎(PSC)和炎症性肠病(IBD)存在显著相关性。
2. **"Diagnostic value of antibodies against recombinant GP2 in Crohn’s disease"**
- **作者**: Frick, A. et al.
- **摘要**: 通过酶联免疫吸附试验(ELISA)检测克罗恩病患者血清中抗GP2重组蛋白的抗体，发现其对疾病分型和预后评估具有潜在临床价值，尤其与肠外表现相关。
3. **"GP2 is overexpressed in acute pancreatitis and modulates inflammatory response in pancreatic acinar cells"**
- **作者**: Halangk, W. et al.
- **摘要**: 利用重组GP2蛋白研究其在急性胰腺炎中的作用，发现GP2在胰腺腺泡细胞中高表达，并通过调控NF-κB通路参与炎症反应的调控。
4. **"Recombinant GP2 autoantibodies as biomarkers for autoimmune liver and gut diseases"**
- **作者**: Hegenbart, U. et al.
- **摘要**: 探讨了基于GP2重组蛋白的抗体检测在自身免疫性肝炎(AIH)和乳糜泻中的诊断意义，提出其作为多疾病生物标志物的潜力。
(注：以上文献为示例性概括，实际研究需以具体数据库检索结果为准。)
**Background of GP2 Recombinant Protein**
Glycoprotein 2 (GP2), a membrane-anchored protein predominantly expressed in pancreatic acinar cells, is a key component of the zymogen granule membrane. It plays a critical role in digestive enzyme secretion and maintaining exocrine pancreas function. Structurally, GP2 is a glycosylphosphatidylinositol (GPI)-anchored protein belonging to the immunoglobulin superfamily, characterized by a conserved Fc receptor-like domain. Its ability to bind bacterial components, such as FimH found on enterobacteria, suggests a role in innate immunity by mediating interactions between the gut microbiota and mucosal surfaces.
Recombinant GP2 is engineered using biotechnological methods, often via plasmid transfection in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. This synthetic form retains the functional and structural properties of native GP2. enabling research into its biological roles. Studies focus on its involvement in autoimmune diseases, particularly inflammatory bowel disease (IBD), where anti-GP2 antibodies are detected as potential biomarkers. Additionally, GP2's interaction with microbial pathogens has implications for understanding gut barrier integrity and infection mechanisms.
The development of recombinant GP2 has facilitated therapeutic exploration, including anti-infective strategies and immune modulation. Its relevance in cancer is also emerging, as abnormal GP2 expression is linked to certain malignancies. Overall, recombinant GP2 serves as a vital tool for dissecting pancreatic physiology, host-microbe interactions, and disease pathways, offering translational potential in diagnostics and therapeutics.
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