| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANK1 |
| Uniprot No | P16157 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANK1重组蛋白的3篇参考文献(信息基于公开研究整理，部分为模拟概括)：
1. **文献名称**：*"Ankyrin-1 mutations cause hereditary spherocytosis by disrupting the stability of erythrocyte membrane"*
**作者**：Bennett, V., et al.
**摘要**：研究揭示了ANK1基因突变导致遗传性球形红细胞症的作用机制，通过重组蛋白实验发现ANK1缺陷会破坏细胞膜骨架与膜蛋白的连接，导致红细胞形态异常和溶血。
2. **文献名称**：*"Structural basis of ankyrin-1 function in spectrin binding and membrane domain organization"*
**作者**：Lorenzo, D.N., et al.
**摘要**：利用重组ANK1蛋白进行结构生物学分析，阐明了其与血影蛋白(spectrin)结合的分子机制，并揭示了ANK1在维持细胞膜结构域动态组装中的关键作用。
3. **文献名称**：*"ANK1 interacts with microtubules to regulate neuronal polarity"*
**作者**：Zhang, Y., et al.
**摘要**：研究发现重组ANK1蛋白通过调控微管稳定性参与神经元极性的建立，其功能缺失会导致轴突发育异常，提示ANK1在神经系统疾病中的潜在病理意义。
4. **文献名称**：*"Ankyrin-1 deficiency exacerbates hyperglycemia-induced endothelial dysfunction in diabetes"*
**作者**：Gallagher, P.G., et al.
**摘要**：通过构建ANK1敲除模型及重组蛋白回补实验，发现ANK1通过调控内皮细胞一氧化氮信号通路参与糖尿病血管并发症的发生，为治疗提供了新靶点。
(注：以上文献为示例性质，实际引用时需核对原文准确性。)
**Background of ANK1 Recombinant Protein**
Ankyrin-1 (ANK1) is a cytoskeletal protein critical for maintaining membrane integrity and cellular structure by bridging integral membrane proteins with the underlying spectrin-actin network. It belongs to the ankyrin family, characterized by tandem ankyrin repeats that mediate protein-protein interactions. ANK1 is predominantly expressed in erythrocytes, where it stabilizes the plasma membrane by linking the anion exchanger (band 3) to the spectrin cytoskeleton. Mutations in the *ANK1* gene are associated with hereditary spherocytosis (HS), a hemolytic anemia characterized by fragile, spherical red blood cells.
The recombinant ANK1 protein is engineered using biotechnological methods, often expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. This recombinant form retains functional domains, including the N-terminal membrane-binding domain, the spectrin-binding domain, and the C-terminal regulatory domain. Researchers utilize ANK1 recombinant protein to study its role in erythrocyte mechanics, membrane stability, and interactions with partners like band 3. Na+/K+ ATPase, and neuroglobin.
Beyond erythrocytes, ANK1 is implicated in neuronal and cardiac tissues, suggesting broader roles in cellular signaling and organelle organization. Its recombinant version serves as a tool for diagnostic assays, drug screening for HS, and exploring pathological mechanisms in neurodegenerative and cardiovascular diseases. Studies also leverage ANK1 to investigate its potential as a therapeutic target or biomarker, highlighting its multifaceted importance in basic and applied biomedical research.
In summary, ANK1 recombinant protein provides a versatile platform for dissecting molecular interactions in cellular stability and disease pathogenesis, bridging structural biology with clinical applications.
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