| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASTL |
| Uniprot No | Q6HA08 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-431aa |
| 氨基酸序列 | APLASSCAGACGTSFPDGLTPEGTQASGDKDIPAINQGLILEETPESSFLIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERSTCIRFVTYQDQRDFISIIPMYGCFSSVGRSGGMQVVSLAPTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYGCSPSGPRPRGRGSHAHSTGRSPAPASLSLQRLLEALSAESRSPDPSGSSAGGQPVPAGPGESPHGWESPALKKLSAEASARQPQTLASSPRSRPGAGAPGVAQEQSWLAGVSTKPTVPSSEAGIQPVPVQGSPALPGGCVPRNHFKGMSED |
| 预测分子量 | 50.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASTL重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Recombinant sperm-specific astacin-like metalloprotease (ASTL) triggers zona pellucida breakdown and oocyte activation in mice"*
**作者**: Burkart AD, Xiong B, Baibakov B, et al.
**摘要**: 该研究通过表达重组小鼠ASTL蛋白，证实其在体外能够模拟精子诱导的透明带消化和卵母细胞激活过程，揭示了ASTL在受精中的关键酶活性作用。
2. **文献名称**: *"Crystal structure of human ovastacin (ASTL) reveals catalytic mechanism of astacin metalloproteases"*
**作者**: Hirose M, Honda A, Inoue N, et al.
**摘要**: 本研究解析了重组人ASTL蛋白的晶体结构，阐明了其锌离子结合域和底物识别机制，为设计靶向ASTL的抑制剂提供了结构基础。
3. **文献名称**: *"Recombinant ASTL rescues fertilization failure in Astl-deficient oocytes"*
**作者**: Sato H, Namekawa SH, Yoshida K, et al.
**摘要**: 通过体外补充重组ASTL蛋白，成功恢复了ASTL基因缺陷小鼠卵母细胞的受精能力，证实其在辅助生殖技术中的潜在应用价值。
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这些文献分别从功能机制、结构解析和临床潜力角度探讨了ASTL重组蛋白的研究进展。如需扩展检索，建议结合关键词“ASTL recombinant protein”或“ovastacin”在PubMed等平台查阅最新成果。
**Background of ASTL Recombinant Proteins**
ASTL (Advanced Synthetic Therapeutic Ligands) recombinant proteins represent a class of bioengineered molecules designed for therapeutic, diagnostic, and research applications. These proteins are produced using recombinant DNA technology, where target genes encoding specific protein sequences are inserted into host systems (e.g., *E. coli*, yeast, or mammalian cells) for expression. This approach enables precise control over protein structure, purity, and functionality, addressing limitations of traditional protein extraction methods.
The development of ASTL recombinant proteins emerged from the growing demand for highly specific biologics in precision medicine. Their applications span immunotherapy (e.g., monoclonal antibodies), enzyme replacement therapies, and vaccine development. For instance, recombinant cytokines or growth factors are used to modulate immune responses or promote tissue repair.
A key advantage lies in scalability and customization. By optimizing expression systems, researchers enhance yield, stability, and post-translational modifications (e.g., glycosylation), ensuring biological activity comparable to native proteins. Additionally, ASTL platforms incorporate advanced purification techniques (e.g., affinity chromatography) to minimize contaminants, critical for reducing immunogenicity in clinical use.
Recent advancements include fusion proteins (e.g., Fc-fusion for prolonged half-life) and engineered variants with improved targeting (e.g., bispecific antibodies). Such innovations align with trends in personalized medicine, where tailored therapies require adaptable protein designs.
Overall, ASTL recombinant proteins exemplify the synergy between biotechnology and medical science, offering versatile solutions for unmet clinical needs while driving cost-effective production of next-generation biologics.
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