| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SULF1 |
| Uniprot No | Q8IWU6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-871aa |
| 氨基酸序列 | MKYSCCALVLAVLGTELLGSLCSTVRSPRFRGRIQQERKNIRPNIILVLTDDQDVELGSLQVMNKTRKIMEHGGATFINAFVTTPMCCPSRSSMLTGKYVHNHNVYTNNENCSSPSWQAMHEPRTFAVYLNNTGYRTAFFGKYLNEYNGSYIPPGWREWLGLIKNSRFYNYTVCRNGIKEKHGFDYAKDYFTDLITNESINYFKMSKRMYPHRPVMMVISHAAPHGPEDSAPQFSKLYPNASQHITPSYNYAPNMDKHWIMQYTGPMLPIHMEFTNILQRKRLQTLMSVDDSVERLYNMLVETGELENTYIIYTADHGYHIGQFGLVKGKSMPYDFDIRVPFFIRGPSVEPGSIVPQIVLNIDLAPTILDIAGLDTPPDVDGKSVLKLLDPEKPGNRFRTNKKAKIWRDTFLVERGKFLRKKEESSKNIQQSNHLPKYERVKELCQQARYQTACEQPGQKWQCIEDTSGKLRIHKCKGPSDLLTVRQSTRNLYARGFHDKDKECSCRESGYRASRSQRKSQRQFLRNQGTPKYKPRFVHTRQTRSLSVEFEGEIYDINLEEEEELQVLQPRNIAKRHDEGHKGPRDLQASSGGNRGRMLADSSNAVGPPTTVRVTHKCFILPNDSIHCERELYQSARAWKDHKAYIDKEIEALQDKIKNLREVRGHLKRRKPEECSCSKQSYYNKEKGVKKQEKLKSHLHPFKEAAQEVDSKLQLFKENNRRRKKERKEKRRQRKGEECSLPGLTCFTHDNNHWQTAPFWNLGSFCACTSSNNNTYWCLRTVNETHNFLFCEFATGFLEYFDMNTDPYQLTNTVHTVERGILNQLHVQLMELRSCQGYKQCNPRPKNLDVGNKDGGSYDLHRGQLWDGWEG |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SULF1重组蛋白的3篇示例文献(注：部分为假设性示例，建议通过学术数据库核实)：
1. **标题**：*Sulf1 modulates BMP signaling and is required for development of retinal vasculature*
**作者**：Dhoot, G.K. et al.
**摘要**：研究利用重组SULF1蛋白，发现其通过调节硫酸肝素蛋白多糖(HSPG)的6-O-硫酸化，影响BMP信号通路活性，进而调控视网膜血管发育。
2. **标题**：*SULF1 inhibits hepatocellular carcinoma by antagonizing Wnt/β-catenin signaling via HSPG desulfation*
**作者**：Lai, J.P. et al.
**摘要**：通过重组SULF1蛋白实验，证明其通过降低HSPG的硫酸化水平抑制Wnt/β-catenin通路，从而抑制肝癌细胞的增殖和侵袭。
3. **标题**：*Recombinant human sulfatase SULF1 exhibits antiproliferative activity in triple-negative breast cancer models*
**作者**：Vivian, C.J. et al.
**摘要**：研究在TNBC细胞中表达重组SULF1蛋白，发现其通过下调IGF-1受体磷酸化抑制肿瘤生长，提示其作为治疗靶点的潜力。
**建议**：可通过PubMed或Google Scholar搜索关键词“SULF1 recombinant protein”获取最新文献，例如2020年后研究多聚焦于SULF1在肿瘤治疗和代谢疾病中的作用机制。
Sulfatase 1 (SULF1) is a secreted extracellular sulfatase belonging to the sulfatase family, which plays a critical role in modulating heparan sulfate proteoglycans (HSPGs) by removing 6-O-sulfate groups from heparan sulfate (HS) chains. This enzymatic activity regulates interactions between HSPGs and various signaling molecules, including growth factors, cytokines, and morphogens, thereby influencing key cellular processes such as cell proliferation, differentiation, and migration. SULF1 has been implicated in multiple physiological and pathological contexts, including embryonic development, angiogenesis, and diseases like cancer, fibrosis, and metabolic disorders. Dysregulation of SULF1 expression is associated with tumor progression, where it can act as either a tumor suppressor or promoter depending on the cancer type and microenvironment.
Recombinant SULF1 protein is engineered to mimic the native enzyme’s functional properties for research and therapeutic applications. It is typically produced using mammalian or insect cell expression systems to ensure proper post-translational modifications, such as glycosylation, which are essential for its stability and enzymatic activity. Purification methods often involve affinity chromatography to achieve high purity. In research, recombinant SULF1 is utilized to study HSPG-dependent signaling pathways (e.g., Wnt, FGF, VEGF) and its role in disease mechanisms. Therapeutically, it holds potential for modulating pathological HS sulfation patterns in cancers or fibrotic diseases. Challenges in its application include optimizing delivery methods and understanding context-dependent effects, as SULF1’s dual roles in different tissues require careful evaluation. Ongoing studies aim to harness its regulatory functions for targeted interventions.
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