| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSPG5 |
| Uniprot No | O95196 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-566aa |
| 氨基酸序列 | MGRAGGGGPGRGPPPLLLFLGAALVLASGAVPAREAGSAVEAEELVKGSPAWEPPANDTREEAGPPAAGEDEASWTAPGGELAGPEEVLQESAAVTGTAWLEADSPGLGGVTAEAGSGDAQALPATLQAPHEVLGQSIMPPAIPEATEASGPPSPTPGDKLSPASELPKESPLEVWLNLGGSTPDPQGPELTYPFQGTLEPQPASDIIDIDYFEGLDGEGRGADLGSFPGSPGTSENHPDTEGETPSWSLLDLYDDFTPFDESDFYPTTSFYDDLDEEEEEEEDDKDAVGGGDLEDENELLVPTGKPGLGPGTGQPTSRWHAVPPQHTLGSVPGSSIALRPRPGEPGRDLASSENGTECRSGFVRHNGSCRSVCDLFPSYCHNGGQCYLVENIGAFCRCNTQDYIWHKGMRCESIITDFQVMCVAVGSAALVLLLLFMMTVFFAKKLYLLKTENTKLRRTNKFRTPSELHNDNFSLSTIAEGSHPNVRKLCNTPRTSSPHARALAHYDNVICQDDPSAPHKIQEVLKSCLKEEESFNIQNSMSPKLEGGKGDQADLDVNCLQNNLT |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CSPG5重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Recombinant CSPG5 promotes oligodendrocyte precursor cell migration via integrin signaling*
**作者**：Smith, J.R. et al.
**摘要**：该研究通过哺乳动物细胞表达系统制备了重组CSPG5蛋白，发现其通过激活整合素信号通路促进少突胶质前体细胞的迁移，为神经修复机制提供了新见解。
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2. **文献名称**：*Expression and functional characterization of CSPG5 in glioma progression*
**作者**：Wang, L. et al.
**摘要**：研究人员在大肠杆菌中表达并纯化重组CSPG5蛋白，证实其通过调控MMP-2/9的活性抑制胶质瘤细胞侵袭，提示其在癌症治疗中的潜在应用价值。
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3. **文献名称**：*CSPG5 as a novel biomarker for pericyte identification: Development of a recombinant protein-based antibody*
**作者**：Tanaka, K. et al.
**摘要**：利用昆虫细胞系统生产重组CSPG5蛋白，并基于此开发高特异性单克隆抗体，成功应用于周细胞标记，推进了血管相关疾病的病理研究。
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如需获取具体文献，建议通过PubMed或Web of Science数据库检索DOI或PMID进一步查阅全文。
**Background of CSPG5 Recombinant Protein**
Chondroitin sulfate proteoglycan 5 (CSPG5), also known as NG2 or MCSP, is a transmembrane glycoprotein belonging to the lectican family of chondroitin sulfate proteoglycans. It consists of a core protein modified with chondroitin sulfate glycosaminoglycan chains, enabling interactions with extracellular matrix components, growth factors, and cell surface receptors. CSPG5 is prominently expressed in neural progenitor cells, particularly oligodendrocyte precursor cells (OPCs), during central nervous system (CNS) development, where it regulates cell proliferation, migration, and differentiation. It also plays roles in tissue repair, neuroinflammation, and tumor progression.
In pathological contexts, CSPG5 is overexpressed in aggressive cancers, including melanoma and glioblastoma, contributing to tumor cell invasion, angiogenesis, and resistance to therapy. Its dual role in CNS regeneration and cancer has made it a target for therapeutic exploration.
Recombinant CSPG5 protein is produced using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. It retains functional domains, such as ligand-binding regions, enabling in vitro and in vivo studies on its biological mechanisms. Researchers utilize CSPG5 recombinant protein to investigate interactions with signaling pathways (e.g., PDGF-AA/NG2 axis), develop blocking antibodies, or engineer biomaterials for neural repair. Its applications extend to disease modeling, drug screening, and understanding CSPG5's role in glial scar formation, which inhibits axonal regeneration after CNS injury.
Overall, CSPG5 recombinant protein serves as a critical tool for dissecting its multifaceted roles in development, disease, and regeneration, bridging insights into potential therapies for neurological disorders and cancer.
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