| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HS6ST3 |
| Uniprot No | Q8IZP7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-471aa |
| 氨基酸序列 | MDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAGEAGPPAVPGPARRAQAPPEEWERRPQLPPPPRGPPEGPRGAAAPEEEDEEPGDPREGEEEEEEDEPDPEAPENGSLPRFVPRFNFSLKDLTRFVDFNIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHADWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDELPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAFFGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYHHTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRW |
| 预测分子量 | 54,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HS6ST3重组蛋白的示例参考文献(内容为模拟概括，仅供参考)：
---
1. **标题**：*Enzymatic Characterization of Recombinant Human HS6ST3 in Heparan Sulfate Sulfation*
**作者**：Smith A, et al.
**摘要**：本研究通过哺乳动物表达系统成功制备了HS6ST3重组蛋白，证实其特异性地催化硫酸肝素(HS)6-O位点的磺酸化反应，并揭示其对不同糖链底物的选择性修饰，为研究HS6ST3在细胞信号通路中的作用提供工具。
2. **标题**：*HS6ST3 Recombinant Protein Promotes Tumor Angiogenesis via VEGF Signaling*
**作者**：Zhang Y, et al.
**摘要**：通过体外实验发现，HS6ST3重组蛋白通过增强硫酸肝素对VEGF的亲和力，促进血管内皮细胞增殖和肿瘤血管生成，提示HS6ST3可能成为癌症治疗的潜在靶点。
3. **标题**：*Structural Insights into HS6ST3 Catalytic Mechanism by X-ray Crystallography*
**作者**：Tanaka K, et al.
**摘要**：首次解析了HS6ST3重组蛋白的晶体结构，揭示了其底物结合口袋的关键氨基酸残基及催化磺酸基转移的分子机制，为设计选择性抑制剂奠定结构基础。
4. **标题**：*HS6ST3 Knockdown and Recombinant Rescue in Neural Development Models*
**作者**：Lee S, et al.
**摘要**：利用HS6ST3重组蛋白回补实验，证明其在神经突触形成中通过调控Slit-Robo信号通路影响轴突导向，强调了6-O硫酸化修饰对神经系统发育的重要性。
---
**备注**：以上文献为示例，实际研究请通过PubMed、Google Scholar等平台以“HS6ST3 recombinant protein”或“HS6ST3 sulfotransferase”为关键词检索最新成果。
Heparan sulfate 6-O-sulfotransferase 3 (HS6ST3) is a member of the heparan sulfate sulfotransferase family, which plays a critical role in modifying heparan sulfate (HS) proteoglycans. HS chains are linear polysaccharides attached to core proteins, functioning as key regulators of cell signaling, extracellular matrix interactions, and molecular recognition. HS6ST3 specifically catalyzes the transfer of sulfate groups to the 6-O position of glucosamine residues within HS, influencing the structural diversity and functional specificity of these glycans. This enzymatic modification is essential for mediating ligand-receptor interactions, including growth factors (e.g., FGFs, VEGF) and morphogens, thereby regulating developmental processes, cell proliferation, and tissue homeostasis.
The HS6ST3 gene is located on human chromosome 13q31.3 and is expressed in various tissues, with notable activity in the brain, pancreas, and reproductive organs. Its recombinant protein form is engineered for in vitro studies to investigate HS sulfation patterns, enzymatic mechanisms, and roles in disease contexts. Dysregulation of HS6ST3 has been linked to pathological conditions such as cancer, metabolic disorders, and neurological abnormalities. For instance, altered HS6ST3 expression may affect tumor angiogenesis or insulin signaling pathways, making it a potential therapeutic target.
Recombinant HS6ST3 is typically produced using mammalian or insect cell systems to ensure proper post-translational modifications. It serves as a tool for biochemical assays, structural studies, and drug discovery aimed at modulating HS-dependent pathways. Research on HS6ST3 also contributes to understanding developmental defects and designing glycan-based therapeutics targeting HS-protein interactions.
×
