| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HS6ST2 |
| Uniprot No | Q96MM7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605aa |
| 氨基酸序列 | MALPACAVREFEPPRQPERGAPVRTTCPRRHSRVEAELAASRPGSVAASVRAGPPRGVSHGFHTRPLLDKPRKASSSLAGAACAPLFALLSRGRRRRMHVLRRRWDLGSLCRALLTRGLAALGHSLKHVLGAIFSKIFGPMASVGNMDEKSNKLLLALVMLFLFAVIVLQYVCPGTECQLLRLQAFSSPVPDPYRSEDESSARFVPRYNFTRGDLLRKVDFDIKGDDLIVFLHIQKTGGTTFGRHLVRNIQLEQPCECRVGQKKCTCHRPGKRETWLFSRFSTGWSCGLHADWTELTSCVPSVVDGKRDARLRPSRNFHYITILRDPVSRYLSEWRHVQRGATWKASLHVCDGRPPTSEELPSCYTGDDWSGCPLKEFMDCPYNLANNRQVRMLSDLTLVGCYNLSVMPEKQRNKVLLESAKSNLKHMAFFGLTEFQRKTQYLFEKTFNMNFISPFTQYNTTRASSVEINEEIQKRIEGLNFLDMELYSYAKDLFLQRYQFMRQKEHQEARRKRQEQRKFLKGRLLQTHFQSQGQGQSQNPNQNQSQNPNPNANQNLTQNLMQNLTQSLSQKENRESPKQNSGKEQNDNTSNGTNDYIGSVEKWR |
| 预测分子量 | 69,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HS6ST2重组蛋白的三篇示例参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"Recombinant Expression and Functional Characterization of HS6ST2 in Heparan Sulfate Biosynthesis"*
**作者**：Müller et al.
**摘要**：研究利用昆虫细胞系统成功表达重组HS6ST2蛋白，证实其在硫酸乙酰肝素(HS)6-O位点硫酸化中的关键作用，并发现其活性影响VEGF介导的血管生成。
2. **文献名称**：*"HS6ST2 Recombinant Protein Modulates Wnt Signaling in Stem Cell Differentiation"*
**作者**：Chen et al.
**摘要**：通过体外实验证明，重组HS6ST2通过改变HS链的结构，增强Wnt3a与受体的结合能力，从而调控胚胎干细胞的神经分化路径。
3. **文献名称**：*"Targeting HS6ST2 in Breast Cancer: Recombinant Enzyme Screening for Inhibitor Development"*
**作者**：Garcia-Rojas et al.
**摘要**：利用重组HS6ST2蛋白高通量筛选小分子库，鉴定出选择性抑制剂，可抑制乳腺癌细胞迁移及体内转移，提示其作为治疗靶点的潜力。
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这些示例反映了HS6ST2重组蛋白在酶学机制、信号通路调控及疾病治疗中的研究方向，涵盖表达技术、功能分析和应用开发。实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
Heparan sulfate 6-O-sulfotransferase 2 (HS6ST2) is a member of the sulfotransferase family that catalyzes the transfer of sulfate groups to specific positions on heparan sulfate (HS) chains, a critical post-translational modification influencing HS-protein interactions. HS, a linear polysaccharide, plays pivotal roles in cellular signaling, extracellular matrix organization, and ligand-receptor binding. The 6-O-sulfation mediated by HS6ST2 modulates HS interactions with growth factors (e.g., FGFs, VEGF), morphogens (e.g., Wnt, Hedgehog), and chemokines, thereby regulating developmental processes, angiogenesis, and immune responses. Dysregulation of HS6ST2 expression or activity is implicated in pathologies such as cancer metastasis, metabolic disorders, and neurodevelopmental conditions.
Recombinant HS6ST2 protein is engineered to study its enzymatic mechanisms, substrate specificity, and structural features in vitro. Typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper folding and post-translational modifications, the recombinant protein retains catalytic activity for functional assays. Its applications include elucidating HS sulfation patterns in disease models, screening therapeutic agents targeting HS-dependent pathways, and exploring HS6ST2's role in tissue-specific signaling. Challenges in production include maintaining enzyme stability and optimizing purification protocols to avoid loss of activity. Research leveraging recombinant HS6ST2 has advanced understanding of HS biology, offering potential for therapeutic interventions in HS-related disorders.
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