| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE4D |
| Uniprot No | Q08499 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-507aa |
| 氨基酸序列 | ASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKE KEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNK WGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLED HYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHD VDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQN LTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLD NYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERG MEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLED NREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSG SQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVID DRSPDT |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDE4D重组蛋白的3篇参考文献概览：
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1. **文献名称**：*Structural and Functional Characterization of Recombinant PDE4D: A Therapeutic Target for Cognitive Disorders*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌表达系统成功制备重组PDE4D蛋白，解析其晶体结构，并验证其cAMP水解活性。结果表明，PDE4D的UCR2结构域对酶活性和抑制剂结合具有关键作用，为神经退行性疾病药物开发提供依据。
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2. **文献名称**：*Expression and Purification of Active Human PDE4D Catalytic Domain in Insect Cells*
**作者**：Chen L, et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达人源PDE4D催化结构域重组蛋白，通过亲和层析和凝胶过滤纯化获得高纯度蛋白。功能实验证实该重组蛋白具有与天然蛋白相似的酶动力学特性，适用于高通量药物筛选。
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3. **文献名称**：*Role of PDE4D Isoforms in Airway Inflammation: Insights from Recombinant Protein Studies*
**作者**：Johnson R, et al.
**摘要**：通过构建不同PDE4D亚型(D1/D3)的重组蛋白，比较其对炎症细胞中cAMP信号通路的调控差异。研究发现PDE4D3在巨噬细胞中的选择性高表达可能成为哮喘治疗的潜在靶点。
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**备注**：以上文献为示例，实际引用需根据具体研究方向补充真实文献(可通过PubMed或Web of Science检索关键词“PDE4D recombinant protein”或“PDE4D expression”获取)。
**Background of PDE4D Recombinant Protein**
Phosphodiesterase 4D (PDE4D), a member of the PDE4 subfamily, is an enzyme that hydrolyzes cyclic adenosine monophosphate (cAMP), a critical secondary messenger regulating cellular signaling pathways. PDE4D is widely expressed in tissues, including the central nervous, immune, and cardiovascular systems, and plays a role in processes such as inflammation, neuronal plasticity, and smooth muscle contraction. Its dysregulation has been implicated in neuropsychiatric disorders (e.g., Alzheimer’s disease, depression), chronic obstructive pulmonary disease (COPD), and certain cancers.
Recombinant PDE4D protein is engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functionally active enzyme for research and drug discovery. The recombinant form retains catalytic domains and regulatory regions, enabling studies on its structure, substrate specificity, and interactions with inhibitors. Notably, PDE4D exhibits unique N-terminal regions and splice variants (e.g., PDE4D3. PDE4D5), which influence subcellular localization and protein-protein interactions.
Pharmaceutical interest in PDE4D stems from its potential as a therapeutic target. Selective PDE4 inhibitors, like roflumilast (approved for COPD), target cAMP degradation to modulate immune responses and inflammation. However, subtype-specific inhibition remains challenging due to structural similarities across PDE4 isoforms (A, B, C, D). Recombinant PDE4D facilitates high-throughput screening of selective inhibitors while minimizing off-target effects.
Additionally, recombinant PDE4D is utilized to study cAMP-dependent pathways in disease models, such as neurodegeneration or airway hyperresponsiveness. Its application extends to structural biology (e.g., crystallography) to elucidate inhibitor-binding mechanisms, aiding rational drug design.
In summary, PDE4D recombinant protein serves as a vital tool for understanding cAMP signaling, validating therapeutic targets, and accelerating the development of PDE4D-modulating drugs with improved specificity and efficacy.
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