| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE12 |
| Uniprot No | Q6L8Q7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-609aa |
| 氨基酸序列 | MWRLPGARAA LRVIRTAVEK LSRAEAGSQT AAGAMERAVV RCVPSEPKLS LSFALADGSH KNMQRDQSEP LGRVLSRIAT NALKGHAKAA AAKKSRKSRP NASGGAACSG PGPEPAVFCE PVVKLYYREE AVAEDVLNVD AWQDGAVLQI GDVKYKVERN PPAFTELQLP RYIMAGFPVC PKLSLEFGDP ASSLFRWYKE AKPGAAEPEV GVPSSLSPSS PSSSWTETDV EERVYTPSNA DIGLRLKLHC TPGDGQRFGH SRELESVCVV EAGPGTCTFD HRHLYTKKVT EDALIRTVSY NILADTYAQT EFSRTVLYPY CAPYALELDY RQNLIQKELT GYNADVICLQ EVDRAVFSDS LVPALEAFGL EGVFRIKQHE GLATFYRKSK FSLLSQHDIS FYEALESDPL HKELLEKLVL YPSAQEKVLQ RSSVLQVSVL QSTKDSSKRI CVANTHLYWH PKGGYIRLIQ MAVALAHIRH VSCDLYPGIP VIFCGDFNST PSTGMYHFVI NGSIPEDHED WASNGEEERC NMSLTHFFKL KSACGEPAYT NYVGGFHGCL DYIFIDLNAL EVEQVIPLPS HEEVTTHQAL PSVSHPSDHI ALVCDLKWK |
| 预测分子量 | 67,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDE12重组蛋白的3篇参考文献及其摘要内容的简要概括：
1. **文献名称**：*Structural and functional characterization of recombinant human PDE12*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组人源PDE12蛋白，解析其晶体结构，揭示其底物结合域特征，验证其RNA水解酶活性依赖锌离子。
2. **文献名称**：*PDE12 regulates antiviral innate immunity by degrading mitochondrial RNA*
**作者**：Li X, et al.
**摘要**：发现PDE12重组蛋白通过切割线粒体RNA调控I型干扰素信号通路，敲除PDE12增强细胞抗病毒反应，提示其在先天免疫中的潜在作用机制。
3. **文献名称**：*Development of a high-throughput screening assay for PDE12 inhibitors*
**作者**：Brown K, et al.
**摘要**：基于重组PDE12蛋白建立荧光底物检测体系，筛选小分子抑制剂库，发现多个先导化合物，为抗病毒或神经退行性疾病药物研发提供基础。
(注：以上文献为示例，实际引用需依据具体研究补充真实信息。)
Phosphodiesterase 12 (PDE12), also known as 2',5'-oligoadenylate synthetase-interacting protein, is a member of the phosphodiesterase enzyme family that hydrolyzes cyclic nucleotides, particularly 2',5'-oligoadenylates (2-5A) and cyclic adenosine monophosphate (cAMP). Initially identified for its interaction with components of the innate immune response, PDE12 plays a regulatory role in RNA metabolism and antiviral pathways. It is ubiquitously expressed, with higher activity observed in tissues like the liver, kidney, and brain. Structurally, PDE12 contains a conserved PDE domain responsible for its catalytic activity and a unique N-terminal domain that may mediate protein-protein interactions.
Recombinant PDE12 protein is produced using heterologous expression systems, such as *E. coli* or mammalian cells, to enable functional and structural studies. Its recombinant form retains enzymatic activity, allowing researchers to investigate substrate specificity, inhibition mechanisms, and its role in cellular processes. PDE12's ability to degrade 2-5A, which activates RNase L during viral infection, links it to the regulation of antiviral responses and RNA turnover. Dysregulation of PDE12 has been implicated in pathologies, including neurodegenerative disorders and viral persistence, making it a potential therapeutic target.
Recent studies highlight PDE12's involvement in mitochondrial RNA (mtRNA) processing, suggesting a role in maintaining mitochondrial function. Inhibitors of PDE12 are being explored for modulating immune responses or combating viruses like hepatitis C. The availability of recombinant PDE12 facilitates drug discovery, structural biology (e.g., X-ray crystallography), and mechanistic studies to clarify its physiological and pathological significance. Overall, PDE12 represents a critical enzyme at the intersection of nucleotide metabolism, antiviral defense, and cellular homeostasis.
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